中国现代医学杂志2018，Vol.28Issue(11)：27-32,6.DOI:10.3969/j.issn.1005-8982.2018.011.006
TGF-β1对γδT细胞TLR-4受体及IL-2、IFN-γ水平的影响
TGF-β1 down-regulates expression of TLR-4 and reduces secretion of IL-2 and IFN-γ in γδT cells
杜发旺 1刘红梅 1何正光 1杜先智 2罗晓斌1
作者信息
- 1. 四川省遂宁市中心医院 呼吸内科,四川 遂宁 629000
- 2. 重庆医科大学附属第二医院 呼吸内科,重庆 400010
- 折叠
摘要
Abstract
Objective To research the influence of exogenous TGF-β1on the expression of TLR-4 and inflammatory cytokines (IL-2 and IFN-γ) in the γδT cells. Methods Peripheral γδT cells were collected from healthy human blood, the exogenous TGF-β1and LPS were co-cultured with γδT cells which were divided into four groups including blank control group, LPS group, LPS+TGF-β1group and TGF-β1group. After 12 days the expressions of TLR-4 were detected by PCR and Western blot, the expression levels of IL-2 and IFN-γ were determined by ELISA. Results Compared with the blank control group, the expressions of TLR-4 and the levels of IL-2 and IFN-γ were markedly higher in the LPS and LPS+TGF-β1groups (P < 0.05), but the expressions of TLR-4 and IL-2 were lower in the TGF-β1group (P < 0.05). Compared with the LPS group, the expressions of TLR-4 and the levels of IL-2 and IFN-γ were significantly lower in the LPS+TGF-β1group (P < 0.05). Conclusions TGF-β1can down-regulate the expression of TLR-4 protein and reduce the secretion of IL-2 and IFN-γ in γδT cells.关键词
TGF-β1/γδT淋巴细胞/TLR-4/IL-2/IFN-γKey words
TGF-β1/γδT cell/TLR-4/IL-2/IFN-γ分类
医药卫生引用本文复制引用
杜发旺,刘红梅,何正光,杜先智,罗晓斌..TGF-β1对γδT细胞TLR-4受体及IL-2、IFN-γ水平的影响[J].中国现代医学杂志,2018,28(11):27-32,6.
