中国畜牧杂志2018，Vol.54Issue(3)：9-14,6.DOI:10.19556/j.0258-7033.2018-03-009
CRISPR/cas系统及其在家禽上的应用研究进展
Research Progress on CRISPR/cas System and Application on Poultry Science
摘要
Abstract
CRISPR/cas (Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) is a adaptive immunity system,which widely exists in bacteria and archaea.Among which,Streptococcus pyogenes cas9 (Spcas9) as the most widely known and applyed in different fields of life science because of its high nuclease activity and broad targeting range.While there are some drawbacks limit its application such as large molecular weight and potential off-target effect.As CRISPR/cas system technology developing,some newly discovered effectors were found,The aim of this review article is to describe in detail about research process of CRISPR/cas System including classification,mechanism,newly discovered CRISPR RNA-guided effectors,cas9 variants based on Spcas9,and further review some applications on poultry science.关键词
CRISPR/cas/新效应蛋白/Spcas9变构体/家禽/anti-CRISPRKey words
CRISPR/cas/New effectors/Cas9 variants/Poultry/Anti-CRISPR分类
农业科技引用本文复制引用
黄玲玲,黄小红,陈黎,卢立志..CRISPR/cas系统及其在家禽上的应用研究进展[J].中国畜牧杂志,2018,54(3):9-14,6.基金项目
水禽种质资源培育与创新利用(2015BAD03B06) （2015BAD03B06）
浙江省农业(畜禽)新品种选育重大科技专项(2016C02054-12) （畜禽）
