中国畜牧杂志2018，Vol.54Issue(3)：92-98,7.DOI:10.19556/j.0258-7033.2018-03-092
外源粪菌干预对受体猪肠道屏障功能的影响研究
Effect of Exogenous Fecal Microbiota Intervention on Intestinal Barrier Function in Recipient Pigs
摘要
Abstract
To study the effect of exogenous fecal microbiota intervention on intestinal barrier function in recipient pigs,twelue litters of "Duroc×Landrace×Yorkshire" suckling piglets were randomly allocated into two groups on the basis of similar body weight,birth time and parity.Each group had six litters (10～12 piglets/litter).All piglets were infused by gavage with exogenous fecal suspension or same amount of sterile phosphate buffered solution (PBS) every other day from the first day after birth to 10 days of age.All piglets in each litter had free access to sow and water during the experiment.Piglets were killed and samples were collected on 12 days of age and 27 days of age.The results indicated that:Exogenous fecal microbiota intervention significantly decreased the incidence of diarrhea and increased the average daily gain and survival rate in suckling piglets (P＜0.05).Exogenous fecal microbiota intervention significantly decreased crypt depth in the intestine of suckling piglets at 27 days of age (P＜0.05) and increased villus height/crypt depth in jejunum and ileum at 12 and 27 days of age (P＜0.05).Exogenous fecal microbiota intervention significantly increased the number of goblet cells and SIgA+ cells (12 days of age) and improved the relative expression of Muc2 and tight junction protein ZO-1 and Occludin in the intestine (P＜0.05).While the number of SIgA+ cells had no significant difference between the treatment group and the control group at 27 days of age (P＞0.05).It is suggested that exogenous fecal microbiota intervention can increase the number of goblet cells and SIgA+ cells,improve the expression of Muc2 and tight junction protein and regulate intestinal barrier function in recipient pigs.Exogenous fecal microbiota intervention has the prospect of maintaining intestinal physiology homeostasis and promoting intestinal health in pigs.关键词
外源粪菌干预/哺乳仔猪/黏蛋白/紧密连接蛋白/肠屏障功能Key words
Exogenous fecal microbiota intervention/Suckling piglets/Mucin/Tight junction protein/Intestinal barrier function分类
农业科技引用本文复制引用
耿世杰,李媛,程赛赛,胡栾莎,岳晓敬,韩新燕..外源粪菌干预对受体猪肠道屏障功能的影响研究[J].中国畜牧杂志,2018,54(3):92-98,7.基金项目
浙江省自然科学基金重点项目(LZ18C170001) （LZ18C170001）
