中国药科大学学报2017，Vol.48Issue(6)：680-686,7.DOI:10.11665/j.issn.1000-5048.20170608
多糖锚定修饰紫杉醇-阿霉素复方脂质体的制备及体外评价
Preparation and in vitro studies of polysaccharide modified compound liposomes loaded with paclitaxel and doxorubicin
郝静梅 1王卉 1李琼 2朱靓 1张勇 2霍美蓉2
作者信息
- 1. 中国药科大学制药有限公司,南京210009
- 2. 中国药科大学天然药物活性组分与药效国家重点实验室,南京210009
- 折叠
摘要
Abstract
The objectives of this study were to prepare polysaccharide modified compound liposomes loaded with paclitaxel (PTX) and doxorubicin (DOX) and characterize their phyisicochemical properties,stability and in vitro release profiles.Both PTX-DOX-Lipo and N-lauryl-O-glycol chitosan (LGC) modified liposomes (PTX-DOX-Lipo-LGC) were successfully prepared,and the morphology of the liposomes was observed by transmission electron microscope (TEM),and particle size and zeta potential were analyzed by dynamic light scattering (DLS).pH and osmotic pressure were also determined.The drug loading and encapsulation efficiency,stability and in vitro release were assayed using high-performance liquid phase.Both PTX-DOX-Lipo and PTX-DOX-Lipo-LGC exhibited spherical shape with smooth surface.The average diameter was about 150 nm.pH value and osmotic pressure were in the range of 5.3-6.1 and 820-870 mOsm/kg,respectively.Both PTX and DOX could be encapsulated in liposomes with high encapsulation efficiency (greater than 90％).Compared with PTX-DOX-Lipo,PTX-DOX-Lipo-LGC exhibited lower leakage,higher stability in serum and more sustained release profiles.Moreover,a quicker release rate was observed in pH 5.8 PBS compared with pH 7.4 PBS.PTX-DOX-Lipo-LGC with high drug loading,good stability and sustained drug release profiles has a wide prospect in future clinical application.关键词
紫杉醇-阿霉素复方脂质体/壳聚糖两亲性衍生物/理化性质/稳定性/体外释放Key words
compound liposome loaded with paclitaxel and doxorubicin/amphiphilic chitosan derivatives分类
医药卫生引用本文复制引用
郝静梅,王卉,李琼,朱靓,张勇,霍美蓉..多糖锚定修饰紫杉醇-阿霉素复方脂质体的制备及体外评价[J].中国药科大学学报,2017,48(6):680-686,7.
