中国药房2017，Vol.28Issue(36)：5123-5126,4.DOI:10.6039/j.issn.1001-0408.2017.36.22
HPLC法同时测定腰痛胶囊中4种成分的含量
Simultaneous Determination of 4 Constituents in Yaotong Capsules by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin,cinnamaldehyde,psoralen and isopsoralen in Yaotong capsules.METHODS:HPLC method was adopted.The determination was performed on Agilent-C18 column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelengths were set at 240 nm (paeoniflorin,psoralen,isopsoralen) and 290 nm (cinnamaldehyde).The column temperature was 35 ℃,and sample size was 10 μL.RESULTS:The linear ranges of paeoniflorin,cinnamaldehyde,psoralen and isopsoralen were 0.004 02-0.100 6 mg/mL(r=0.999 8),0.024 50-0.612 7 mg/mL(r=0.999 8),0.005 24-0.131 0 mg/mL(r=0.999 9),0.005 11-0.127 8 mg/mL(r=0.999 9),respectively.LOQ were 37.17,1.47,21.76,25.57 ng,respectively;LOD were 11.15,0.44,6.53,7.67 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 99.02％-100.83％ (RSD=0.68％,n=6),98.58％-100.99％ (RSD=0.83％,n=6),99.78％-101.69％ (RSD=0.89％,n=6),100.06％-102.46％ (RSD=0.92％,n=6),respectively.CONCLUSIONS:The method is simple,accurate and suitable for simultaneous determination of paeoniflorin,cinnamaldehyde,psoralen and isopsoralen in Yaotong capsules.关键词
腰痛胶囊/芍药苷/桂皮醛/补骨脂素/异补骨脂素/高效液相色谱法/含量测定Key words
Yaotong capsule/Paeoniflorin/Cinnamaldehyde/Psoralen/Isopsoralen/HPLC/Content determination分类
医药卫生引用本文复制引用
罗春颖,黄晓婧,史立川,蒲旭峰..HPLC法同时测定腰痛胶囊中4种成分的含量[J].中国药房,2017,28(36):5123-5126,4.基金项目
国家药品标准提高研究课题(No.2015-26) （No.2015-26）
