中国药房2017，Vol.28Issue(36)：5140-5143,4.DOI:10.6039/j.issn.1001-0408.2017.36.26
NIRS结合PLS算法快速测定西洋参饮片中人参皂苷Rg1、Re、Rb1的总含量
Rapid Determination of Ginsenoside Rg1, Re, Rb1 in Panax quinquefolius Pieces by NIRS Combined with PLS Algorithm
摘要
Abstract
OBJECTIVE:To establish the method for rapid determination of ginsenoside Rg1,Re,Rb1 in Panax quinquefolius crude slices.METHODS:HPLC method was adopted to determine the total contents of ginsenoside Rg1,Re,Rb1 (as reference value).NIRS combined PLS algorithm were adopted to establish total quantitative correction model of ginsenoside Rg1,Re,Rb1.According to the reference,62 samples were collected.The spectrum was pretreated with multivariate scattering correction method combined with first order derivative method.The optimal ranges of wave band for ginsenoside Rg1,Re,Rb1 were 7 664.23-5 236.05 cm-1.RESULTS:Methodology validation for total content determination of ginsenoside Rg1,Re,Rb1 was in line with the requirements.For total quantitative correction model of ginsenoside Rg1,Re,Rb1,related correction set coefficient was 0.991 03,corrected mean square deviation 0.010 26.CONCLUSIONS:The method is rapid,accurate,simple and free of contamination.It can be used for rapid determination of ginsenoside Rg1,Re,Rb1 in P quinquefolius crude slices.关键词
西洋参/人参皂苷/近红外漫反射外光谱技术/偏最小二乘法Key words
Panax quinquefolius/Ginsenoside/NIRS/PLS分类
医药卫生引用本文复制引用
左春芳,梁雪琪,喻俊峰,吕亚新,张贤良..NIRS结合PLS算法快速测定西洋参饮片中人参皂苷Rg1、Re、Rb1的总含量[J].中国药房,2017,28(36):5140-5143,4.基金项目
济南军区后勤科研面上项目(No.CJN15J074) （No.CJN15J074）
