中国药房2017，Vol.28Issue(36)：5147-5149,3.DOI:10.6039/j.issn.1001-0408.2017.36.28
HPLC法同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸的含量
Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Fuzi Lizhong Pills (Condensed Pills) by HPLC
方东伟 1彭佳庆1
作者信息
- 1. 鄂州市食品药品检验检测中心,湖北鄂州436000
- 折叠
摘要
Abstract
OBJECTIVE:To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).METHODS:HPLC method was adopted.The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitfile-0.05％ phosphate solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:The linear range ofliquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2).The limits of quantitation were 0.2,0.3 μg/mL,and the limits of detection were 0.1,0.01 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The average recoveries were 98.9％-101.2％ (RSD=0.62％,n=9),98.6％-101.5％ (RSD=1.06％,n=9).CONCLUSIONS:The method is simple and accurate,and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).关键词
附子理中丸(浓缩丸)/高效液相色谱法/甘草苷/甘草酸/含量测定Key words
Fuzi lizhong pills (condensed pills)/HPLC/Liquiritin/Glycyrrhizic acid/Content determination分类
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方东伟,彭佳庆..HPLC法同时测定附子理中丸(浓缩丸)中甘草苷和甘草酸的含量[J].中国药房,2017,28(36):5147-5149,3.
