中国药房2017，Vol.28Issue(36)：5163-5166,4.DOI:10.6039/j.issn.1001-0408.2017.36.33
HPLC-MS/MS法测定桑菊感冒丸中3种成分的含量
Content Determination of 3 Components in Sangju Ganmao Pills by HPLC-MS/MS
刘世军 1孙克明 1刘宪勇 1张敏 1谷雨 1王岩 1毕文超 1魏玮2
作者信息
- 1. 武警山东省总队医院药剂科,济南250014
- 2. 解放军第81医院,南京210002
- 折叠
摘要
Abstract
OBJECTIVE:To establish the method for simultaneous determinations of rutin,forsythin and platycodin D in Sangju ganmao pills.METHODS:HPLC-MS method was adopted.The determination was performed on Waters Atlantis C18 column with mobile phase consisted of acetonitrile-0.1％ formic acid (gradient elution) at the flow rate of 0.2 mL/min.The column temperature was set 35 ℃,and sample size was 10 μL.The ionization mode was electrospray ion,and the reaction mode was multi-reaction monitoring.By positive ion detection mode,the drying gas and nebuliser gas were all high purity nitrogen.The drying gas temperature was 270 ℃.The drying gas flow rate was 25 L/min.The sheath gas flow rate was 10 L/min.The capillary voltage was 4 500 V.The nozzle voltage was 2 000 V and the scanning time was 0.1 s.RESULTS:The linear range of rutin,forsythin and platycodin D were 0.010 82-2.164 μg/mL (r=0.999 7),0.010 18-2.036 μg/mL (r=0.999 4),0.010 27-2.054 μg/mL (r=0.999 7),respectively The limits of quantification were 1.250,0.260,2.720 ng/mL,and the limits of detection were 0.380,0.078,0.820 ng/mL.RSDs of precision,stability and reproducibility tests were all no more than 3.0％.The recoveries were 97.88％-99.88％ (RSD=0.72％,n=6),98.48％-103.13％ (RSD=1.91％,n=6),98.79％-101.41％ (RSD=1.05％,n=6).CONCLUSIONS:This method is simple,precise,stable and reproducible,and can be used for simultaneous determination of rutin,forsythin and platycodin D in Sangju ganmao pills.关键词
高效液相色谱-串联质谱法/桑菊感冒丸/芦丁/连翘苷/桔梗皂苷D/含量Key words
HPLC-MS/MS/Sangju ganmao pills/Rutin/Forsythin/Platycodin D/Content分类
医药卫生引用本文复制引用
刘世军,孙克明,刘宪勇,张敏,谷雨,王岩,毕文超,魏玮..HPLC-MS/MS法测定桑菊感冒丸中3种成分的含量[J].中国药房,2017,28(36):5163-5166,4.
