中国药房2018，Vol.29Issue(1)：33-37,5.DOI:10.6039/j.issn.1001-0408.2018.01.09
HPLC法同时测定平脏调神颗粒中8种成分的含量
Simultaneous Determination of the Contents of 8 Components in Pingzang Tiaoshen Granule by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of berberine hydrochloride,icariin,acteoside,isoflavone glucoside,paeoniflorin,mangiferin,salvianolic acid B and puerarin in Pingzang tiaoshen granule.METHODS:HPLC method was adopted.The determination was performed on InertSustain C18 column with mobile phase consisted of acetonitrile-0.05mol/L potassium dihydrogen phosphate solution (pH to 3,gradient elution) with the flow rate of 1.0 mL/min.The detection wavelength was set at 250 nm (0-23 min,puerarin,mangiferin),230 nm (＞23-30 min,paeoniflorin),220 nm (＞30-50 min,isoflavone glucoside,acteoside),286 nm (＞50-60 min,salvianolic acid B),265 nm (＞60-75 min,berberine hydrochloride),220 nm (＞75-90 min,icariin).The column temperature was set at 30 ℃,and sample size was 10 μL.RESLUTS:The linear range of berberine hydrochloride,icariin,acteoside,isoflavone glucoside,paeoniflorin,mangiferin,salvianolic acid B and puerarin were 4.000-400.0,4.843-484.3,0.498-49.8,2.366-236.6,23.26-2 326.0,3.067-306.7,3.629-362.9 μg/mL,48.23-4 823.2 μg/mL(r≥0.999 4),respectively.The limits of detection were 0.02,0.02,0.02,0.02,0.01,0.02,0.01,0.01 μg/mL;the limits of quantitation were 0.07,0.05,0.06,0.05,0.03,0.07,0.02,0.03 μg/mL,respectively.RSDs of precision,stability (24 h)and repetition tests were a11＜2.0％ (n=6).The average recoveries were 95.77％-103.50％ (RSD=0.77％-2.22％,n=6).CONCLUSIONS:Established method can be used for simultaneous determination of 8 components such as berberine hydrochloride in Pingzang tiaoshen granule.关键词
高效液相色谱法/平脏调神颗粒/盐酸小檗碱/淫羊藿苷/毛蕊花糖苷/毛蕊异黄酮葡萄糖苷/芍药苷/芒果苷/丹酚酸B/葛根素/含量测定Key words
HPLC/Pingzang tiaoshen granule/Berberine hydrochloride/Icariin/Acteoside/Isoflavone glucoside/Paeoniflorin/Mangiferin/Salvianolic acid B/Puerarin/Content determination分类
医药卫生引用本文复制引用
程中琴,刘小妹,施崇精,王姗姗,袁强华,宋英..HPLC法同时测定平脏调神颗粒中8种成分的含量[J].中国药房,2018,29(1):33-37,5.基金项目
四川省科技支撑项目(No.2014SZ0140) （No.2014SZ0140）
