中国药房2018，Vol.29Issue(1)：54-57,4.DOI:10.6039/j.issn.1001-0408.2018.01.14
芬戈莫德对肾缺血再灌注损伤模型小鼠的肾保护作用及其机制研究
Protective Effect of Fingolimod on Renal Ischemia Reperfusion Injury Model Mice and Its Mechanism Study
黄倩 1梁青龙 1陈慧勤 1王梅爱 1黄秋虹 1郑丹丹 1林佩璜1
作者信息
- 1. 泉州医学高等专科学校基础医学部生理教研室,福建泉州 362100
- 折叠
摘要
Abstract
OBJECTIVE:To study the protective effect of fingolimod on renal ischemia reperfusion injury (RIRI) model mice and its mechanism.METHODS:A total of 60 mice were randomly divided into sham operation group,model group,fingolimod group (1 mg/kg) and fingolimod+wortmannin group [fingolimod 1 mg/kg+phosphatidylinositol 3-kinase (PI3K) specific blocker wortmarmin 1.4 mg/kg],with 15 mice in each group.Except for sham operation group,RIRI model was induced in other 3 groups,and those model mice were given relevant medicine via caudal vein at once 24 h before surgery.Serum of mice were collected in each group after 24 h perfusion.Serum levels of Scr and BUN were measured by automatic biochemical analyzer.The pathological changes of renal tissue were observed under light microscope.The protein expression of intercellular cell adhesion molecule-1 (ICAM-1),monocyte chemoattractant protein-1 (MCP-1) and phosphorylated protein kinase B (p-Akt) in renal tissue were measured by Western blot assay.RESULTS:Compared with sham operation group,the serum levels of Scr and BUN in model group were increased significantly (P＜0.01).Pathological changes were found in the kidney,and RIRI led to widespread renal tubular epithelial cell injury,apoptosis and inflammatory cells infiltration.The protein expression of ICAM-1 and MCP-1 in renal tissue were increased significantly (P＜0.01),the protein expression of p-Akt was increased slightly (P＞0.05).Compared with model group,other indexes of fingolimod group were improved significantly (P＜0.01) except that the protein expression of p-Akt in renal tissue was increased significantly (P＜0.01).Compared with fingolimod group,above indexes of fingolimod+wortmannin group were reversed (P＜0.05 or P＜0.01).CONCLUSIONS:Fingolimod can obviously ameliorate renal injury induced by RIRI in mice,the mechanism of which may be associated with the activation of PI3K/Akt signaling pathway.关键词
芬戈莫德/肾缺血再灌注损伤/小鼠/磷脂酰肌醇3-激酶/蛋白激酶BKey words
Fingolimod/Renal ischemia reperfusion injury/Mice/Phosphatidylinositol 3-kinase/Phosphorylated protein kinase B分类
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黄倩,梁青龙,陈慧勤,王梅爱,黄秋虹,郑丹丹,林佩璜..芬戈莫德对肾缺血再灌注损伤模型小鼠的肾保护作用及其机制研究[J].中国药房,2018,29(1):54-57,4.
