摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of 3 components in Li'ermian capsule,including berberine hydrochloride,palmatine hydrochloride and cinnamaldehyde.METHODS:HPLC method was adopted.The determination was performed on Agilent Zorbax SB-C18 with mobile phase consisted of acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution (23 ∶ 77,V/V) at the flow rate of 1.0 mL/min.The detection wavelengths were set at 345 nm (0-20 min,berberine hydrochloride,palmatine hydrochloride) and 290 nm (＞20-30 min,cinnamaldehyde),and column temperature was 30 ℃.The sample size was 10 μL.RESULTS:The linear ranges of berberine hydrochloride,palmatine hydrochloride and cinnamaldehyde were 0.036 80-0.736 0,0.016 76-0.335 2,0.004 140-0.082 80 μg (r≥0.999 5).The limits of detection were 0.110 4,0.050 3,0.124 2 ng,and the limits of quantitation were 0.368 0,0.167 6,0.414 0 ng,respectively.RSDs of precision,stability (12 h) and reproducibility tests were all lower than 2.0％ (n=6).The recoveries were 96.16％-99.73％ (RSD=0.99％-1.21％,n=6).CONCLUSIONS:Established method is simple,reproducible and suitable for simultaneous determination of 3 components such as berberine hydrochloride in Li'ermian capsule.关键词
利尔眠胶囊/高效液相色谱法/盐酸小檗碱/盐酸巴马汀/桂皮醛/含量测定Key words
Li'ermian capsule/HPLC/Berberine hydrochloride/Palmatine hydrochloride/Cinnamaldehyde/Content determination分类
医药卫生
