中国药房2018，Vol.29Issue(1)：65-68,4.DOI:10.6039/j.issn.1001-0408.2018.01.17
甘草提取物对雷公藤甲素损伤后人肝L-02细胞中UGT1A、MRP2表达的影响
Effects of Glycyrrhiza uralensis Extract on the Expression of UGT1A and MRP2 in Human Liver L-02 Cells Damaged by Triptolide
摘要
Abstract
OBJECTIVE:To investigate the effects of Glycyrrhiza uralensis extract (GE) on the expression of uridine diphosphate glucuronyltransferase 1A (UGT1A) and multidrug resistance associated protein 2 (MRP2) in human liver L-02 cells damaged by triptolide (TP),and to study attenuated mechanism of G.uralensison for TP.METHODS:The survival rates of L-02 cells were determined by MTT assay after cultured with 0 (blank control),40,80,160 nmol/L TP for 12,18,24 h.L-02 cells were divided into blank control group (blank culture medium),model control group (80 nmol/L TP) and GE pretreatment group (adding 80 nmol/ L TP after pretreated with 30,60,90 mg/L GE for 24 h);after cultured for 18 h,survival rates of L-02 cells were determined by MTT assay.Rifampin (RIF) group (positive control,adding 80 nmoi/L TP after pretreated with 10 μmol/L RIF for 24 h) was added on the basis of the above grouping (GE concentration of 60 mg/L in GE pretreatment group).After cultured for 24 h,the protein expressions of UGT1A and MRP2 were detected.RESULTS:The inhibition effect of TP on cell proliferation was positively correlated with the concentration and the time.Compared with blank control group,cell survival rate of model control group was decreased significantly (P＜0.05),and the protein expression of MRP2 was decreased significantly (P＜0.01).Compared with model control group,cell survival rates of 30,60,90 mg/L GE pretreatment groups were all increased significantly (P＜0.01).The protein expressions of UGT1A and MRP2 were increased significantly in 60 mg/L GE pretreatment group (P＜0.01).CONCLUSIONS:GE pretreatment can relieve TP-induced human liver L-02 cell damage,and its attenuated mechanism may be associated with the increase the expression of UGT1A and MRP2.关键词
甘草提取物/雷公藤甲素/尿苷二磷酸葡萄糖醛酸转移酶1A/多药耐药相关蛋白2/人肝L-02细胞Key words
Glycyrrhiza uralensis extract/Triptolide/Uri-dine diphosphate glucuronyltransferase 1A/Multidrug resistance associated protein 2/Human liver L-02 cells分类
医药卫生引用本文复制引用
张靖,胡骞,谭亲友,朱胜楠..甘草提取物对雷公藤甲素损伤后人肝L-02细胞中UGT1A、MRP2表达的影响[J].中国药房,2018,29(1):65-68,4.基金项目
国家自然科学基金资助项目(No.81360665) （No.81360665）
广西自然科学基金面上项目(No.2014jjAA40030) （No.2014jjAA40030）
广西自然科学基金青年基金资助项目(No.2013GXNSFBA019174) （No.2013GXNSFBA019174）
广西壮族自治区卫生厅中医药攻关课题(No.GZGG13-08) （No.GZGG13-08）
广西医药卫生自筹经费计划课题(No.Z2014318) （No.Z2014318）
