中国药房2018，Vol.29Issue(2)：168-171,4.DOI:10.6039/j.issn.1001-0408.2018.02.06
声光可调-近红外漫反射光谱法快速评价黄芪药材质量
Rapid Quality Evaluation of Astragali Radix by AOTF-NIR
摘要
Abstract
OBJECTIVE:To establish the method for rapid quality evaluation of Astragali Radix.METHODS:The moisture of medicinal material was determined by oven drying method;the content of astragaloside Ⅳ was determined by HPLC-ELSD;the content of isoflavone glucoside was determined by HPLC (as reference value).The partial least squares (PLS) method combined with acousto-optic turnable filter-NIDRS was adopted to build quantitative model of above indexes in Astragali Radix (as predict value).According to reference value,60 batches of sample were collected.The spectra pretreatment was conducted by first derivative method combined with Savitzky golay.The optimal bands of moisture,astragaloside Ⅳ and isoflavone glucoside were 1 100-2 300 nm,1 080-2 160 nm,1 170-2 230 nm,respectively.RESULTS:The content determination of moisture,astragaloside Ⅳ and isoflavone glucoside in samples were all in line with methodology requirements.The corrected mean square root deviation of quantitative model for moisture,astragaloside Ⅳ,calycosin glucoside were 0.132 3,0.006 6,0.002 5,respectively;predicted mean square root deviation were 0.237 1,0.016 3,0.004 7;internal cross validation coefficient of correction set were 0.975 9,0.953 3,0.968 0;internal verification deviation of quantitative model were 1.43％,1.90％,1.84％;external verification deviation were 1.73 ％,2.68 ％,2.71％,respectively.CONCLUSIONS:The method is rapid,accurate,simple,pollution-free,and can be used for rapid quality evaluation of Astragali Radix.关键词
近红外漫反射光谱法/声光可调/黄芪/水分/黄芪甲苷/毛蕊异黄酮葡萄糖苷Key words
NIDRS/Acousto-optic turnable filter/Astragali Radix/Moisture/Astragaloside Ⅳ/Isoflavone glucoside分类
医药卫生引用本文复制引用
任绪华,苏婷,姜文月,李亚东,郑广晶,高陆..声光可调-近红外漫反射光谱法快速评价黄芪药材质量[J].中国药房,2018,29(2):168-171,4.基金项目
国家中药标准化项目(No.ZYBZH-C-JL-26) （No.ZYBZH-C-JL-26）
吉林省科技发展计划项目(No.20150301009YY、YYZX201503) （No.20150301009YY、YYZX201503）
