中国药房2018，Vol.29Issue(2)：194-198,5.DOI:10.6039/j.issn.1001-0408.2018.02.13
UPLC-MS/MS法同时测定人血浆中卡马西平、文拉法辛、罗格列酮和硝苯地平的浓度
Simultaneous Determination of Carbamazepine, Venlafaxine, Rosiglitazone and Nifedipine in Human Plasma by UPLC-MS/MS
摘要
Abstract
OBJECTIVE:To develop a method for simultaneous determination of carbamazepine,venlafaxine,rosiglitazone and nifedipine in human plasma.METHODS:UPLC-MS/MS method was adopted to determine plasma sample after precipitated with methanol (containing 0.1％ formic acid) using pioglitazone hydrochloride as internal standard.The determination was performed on Waters ACQUITY UPLC HSS C18 column with mobile phase consisted of aqueous solution containing 0.01％ formic acid-methanol containing 0.01％ formic acid (gradient elution) at the flow rate of 0.3 mL/min.The column temperature was 50 ℃,and sample size was 5 μL.ESI was used for positive ion scanning by multi reaction monitoring mode.The ion pairs for quantitative analysis were m/z 237.00 to 194.05 (carbamazepine),m/z 278.20 to 58.10 (venlafaxine),m/z 358.08 to 135.04 (rosiglitazone),m/z 347.15 to 315.17 (nifedipine),m/z 357.09 to 134.06 (internal standard).RESULTS:The liner ranges of carbamazepine,venlafaxine hydrochloride,rosiglitazone hydrochloride and nifedipine were 2.00-2 000.00 (r=0.995 9,n=5),2.12-2 120.00(r=0.990 5,n=5),2.00-2 000.00(r=0.991 5,n=5) and 2.04-1 020.00(r=0.991 0,n=5) ng/mL;the minimum detection limits were 0.200,0.106,0.100,1.020 ng/mL,respectively.RSDs of inter-day and intra-day were less than 15％.The absolute values of RE were less than 15％.The extraction recoveries were 65.66％-96.36％ (RSD％ ＜15％,n=5),and matrix effects ranged 80.99％-114.33％.The plasma concentrations of carbamazepine in 2 epileptic patients were (1 500.41 ± 169.11),(1 159.01 ±59.24) ng/mL(RSD were 11.27％,5.60％,n=3).The plasma concentrations of nifedipine in 2 hypertensive patients were (14.68 ±1.92),(21.18 ± 3.59)ng/mL (RSD were 16.98％,13.10％,n=3).CONCLUSIONS:The method is simple,specific,sensitive,accurate and suitable for the monitoring of plasma concentration and pharmacodynamic study of above drugs.关键词
卡马西平/文拉法辛/罗格列酮/硝苯地平/血药浓度/超高效液相色谱-串联质谱法Key words
Carbamazepine/Venlafaxine/Rosiglitazone/Nifedipine/Plasma concentration/UPLC-MS/MS分类
医药卫生引用本文复制引用
王伟,丁仁奎,李清艳,祁妍敏..UPLC-MS/MS法同时测定人血浆中卡马西平、文拉法辛、罗格列酮和硝苯地平的浓度[J].中国药房,2018,29(2):194-198,5.基金项目
中国民用航空总局科技项目(No.MHRD201042) （No.MHRD201042）
中国民用航空局民航安全能力建设资金项目(No.DFS20160604) （No.DFS20160604）
