中国药理学通报2018，Vol.34Issue(4)：501-507,7.DOI:10.3969/j.issn.1001-1978.2018.04.013
大米α-球蛋白肽YGEGSSEEG对TNF-α诱导的血管内皮细胞损伤的影响
Effects of rice peptide YGEGSSEEG on improving endothelial cell injury induced by tumor necrosis factor
摘要
Abstract
Aim To investigate the peptides and its protection for vascular endothelial cells, derived from the absorbed components of rice α-globulin,which was shown to be effective in anti-atherosclerosis. Methods The amino acid sequence was purified by gel chro-matography and RP-HPLC, and determined by ESI/MS. Then the peptide was chemically synthesized. Hu-man umbilical vein endothelial cell injury model was induced by tumor necrosis factor-α. The cell viability was measured by cell counting kit to screen the appro-priate peptide intervention concentration. The apoptotic rate was detected by flow cytometry. Bcl-2, Bax, p-p38, vascular cell adhesion molecule and the protein expression level of NF-κB signaling pathway were de-tected by Western blot and immunofluorescent stai-ning. Results Apoptosis of HUVECs induced by TNF-α was significantly increased by YGEGSSEEG, which also regulated expression of Bcl-2/Bax proteins and inhibited phosphorylation of p38 protein. Besides, the peptide suppressed the production of VCAM-1, ICAM-1 and activation of NF-κB pathway. While it did not significantly improve the oxidative stress response in HUVECs. Conclusion Peptide YGEGSSEEG pro-tects vascular endothelial cells through suppressing ap-optosis and expression of adhesion molecules.关键词
大米α-球蛋白肽/人脐静脉内皮细胞/细胞凋亡/黏附分子/NF-κB信号通路/Bcl-2/Bax蛋白Key words
rice α-globulin peptide/HUVECs/apop-tosis/adhesion molecules/NF-κB signaling pathway/Bcl-2/Bax分类
医药卫生引用本文复制引用
鞠志远,王丽丽,刘丽娅,周闲容,周素梅,佟立涛..大米α-球蛋白肽YGEGSSEEG对TNF-α诱导的血管内皮细胞损伤的影响[J].中国药理学通报,2018,34(4):501-507,7.基金项目
国家自然科学基金资助项目(No 31401505) （No 31401505）
