中国药理学通报2018，Vol.34Issue(4)：508-512,5.DOI:10.3969/j.issn.1001-1978.2018.04.014
腺苷对肝癌细胞自噬和增殖的作用
Effect of adenosine on autophagy and proliferation of hepatocellular carcinoma cells
摘要
Abstract
Aim To investigate the effect of adenosine on the autophagy and proliferation of hepatocellular carcinoma cells, and improve the curative effect of a-denosine on hepatocellular carcinoma. Methods HepG2 cells were incubated with adenosine, CCK-8 method was used to study the changes of cell prolifera-tion,Western blot was used to study the expression of LC3-Ⅱ and LC3-Ⅰ, and MDC staining was used to observe the number of autophagosomes. Results HepG2 cells were incubated with adenosine(1.0~4.0 mmol·L-1) for 48 h,the proliferation of HepG2 cells were detected at the different time points (12,24,48 h),and the result showed the proliferation was signifi-cantly inhibited by adenosine (P < 0.01). HepG2 cells were incubated with adenosine (0.2,0.5,1.0, 2.0,4.0 mmol·L-1) for 24 h,the ratio of LC3-Ⅱ/LC3-Ⅰ decreased significantly in low concentration of adenosine group (0.2, 0.5 mmol·L-1, P <0.05;1.0 mmol·L-1,P<0.01),and the ratio of LC3-Ⅱ/LC3-Ⅰ increased significantly in higher concentration of adenosine group (4.0 mmol·L-1, P <0.05). HepG2 cells were incubated with adenosine(1.0 mmol·L-1) for 24 h, the ratio of LC3-Ⅱ/LC3-Ⅰ de-creased significantly at 6,12 and 24 h detecting point, the number of autophagosomes were reduced, the low-est ratio of LC3-Ⅱ/LC3-Ⅰ and autophagosomes were observed at 12 h detecting point(P<0.01). Conclu-sions Adenosine inhibits the proliferation of hepato-cellular carcinoma cells,the low concentration of aden-osine inhibits the autophagy,while the high concentra-tion of adenosine increases the autophagy, which is of great significance to reduce multi-drug resistance and improve the therapeutic effect of anti-hepatoma drugs.关键词
腺苷/自噬/多药耐药/肝癌/细胞增殖/肿瘤治疗Key words
adenosine/autophagy/multi-drug resist-ance/hepatocellular carcinoma/cell proliferation/tumor therapy分类
医药卫生引用本文复制引用
蒲泽锦,周小涛,李国平,詹浩炼,郭益添,项梦琦,刘丽璇,谭辉,吴灵飞..腺苷对肝癌细胞自噬和增殖的作用[J].中国药理学通报,2018,34(4):508-512,5.基金项目
广东省科技计划资助项目(No 2014A020212284) （No 2014A020212284）
广东省自然科学基金资助项目(No 2014A030313470) （No 2014A030313470）
