中国药理学通报2018，Vol.34Issue(4)：512-516,5.DOI:10.3969/j.issn.1001-1978.2018.04.015
黄芪多糖对糖尿病大鼠肾脏TGF-β1/Smads信号通路的影响
Effects of astragalus polysaccharide on renal TGF-β1 /Smads signaling pathway in rats with diabetes mellitus
摘要
Abstract
Aim To investigate the effects of astragalus polysaccharide (APS) on renal TGF-β1/Smads signa-ling pathway in rats with diabetes mellitus(DM). Methods Wistar rats were randomly divided into nor-mal group,DM group,APS low dose (APS-low) group and APS high dose (APS-high) group. Rats in APS-low group and APS-high group respectively received 200 and 400 mg·kg-1·d-1APS for 8 weeks. Con-centrations of fasting blood-glucose(FBG),blood urea nitrogen and creatinine,as well as urinary kidney injury molecule-1 (KIM-1) and osteopontin (OPN) were measured. Levels of TGF-β1,Smad2,phosphorylated Smad2 (p-Smad2),Smad3,phosphorylated Smad3 (p-Smad3),Smad7,matrix metalloproteinase (MMP) 2,MMP-9,tissue inhibitor of matrix metalloproteinases (TIMP)-1 and TIMP-2 were investigated. Results Compared to control group,DM group had higher levels of FBG,blood urea nitrogen,creatinine KIM-1,OPN, TGF-β1,Smad2,p-Smad2,Smad3,p-Smad3,TIMP-1 and TIMP-2,but lower levels of Smad7,MMP-2 and MMP-9. APS significantly decreased the levels of FBG,blood urea nitrogen,creatinine KIM-1 and OPN, as well as inhibited the activity of TGF-β1/Smads sig-naling pathway. Conclusion The renoprotective effects of APS might be associated with the inhibition of TGF-β1/Smads signaling pathway.关键词
黄芪多糖/糖尿病/肾损伤/TGF-β1/Smads/MMP/TIMPKey words
astragalus polysaccharide/diabetes melli-tus/renal damage/TGF-β1/Smads/MMP/TIMP分类
医药卫生引用本文复制引用
李承德,王煜,曲敬蓉,张晓俊,毛淑梅,聂克..黄芪多糖对糖尿病大鼠肾脏TGF-β1/Smads信号通路的影响[J].中国药理学通报,2018,34(4):512-516,5.基金项目
国家自然科学基金资助项目(No 81673779,81373828) （No 81673779,81373828）
山东省自然科学基金资助项目 (No ZR2009CL045, ZR2011HL066,ZR2013CM033,ZR2015CL029) （No ZR2009CL045, ZR2011HL066,ZR2013CM033,ZR2015CL029）
山东省教育厅科技计划项目(No J15LL54,J16LM11) （No J15LL54,J16LM11）
山东省中医药管理局项目(No 2013-242,2015-237) （No 2013-242,2015-237）
