中国药理学通报2018，Vol.34Issue(4)：537-543,7.DOI:10.3969/j.issn.1001-1978.2018.04.020
佐剂性关节炎大鼠滑膜及血清中MANF的表达及其与炎症的相关性
Expression characterization of MANF during course of rat adjuvant arthritis and its relationship with inflammation
摘要
Abstract
Aim To observe the expression of mesen-cephalic astrocyte-derived neurotrophic factor(MANF) in synovial membrane and serum of rats with adjuvant arthritis (AA) and to analyse the relationship between MANF expression and arthritis. Methods AA models were prepared by injecting Freund complete adjuvant (FCA) into SD rats. The swelling of the secondary joint was measured by foot volume measurement. The severity of AA was recorded by arthritis index (AI). Synovial pathological changes were observed by HE staining. The protein and mRNA levels of MANF,BiP and CHOP extracted from synovial tissues in different periods of AA rats were detected by Western blot and reverse transcription-polymerase chain reaction (RT-PCR), respectively. The levels of MANF, C-reactive protein (CRP), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) in serum were detected by enzyme-linked immunosorbent assay (ELISA) and then the relationship between MANF level and inflam-matory factors were explored. Results AA rat model was established successfully. The expression of BiP significantly increased in synovial tissue on d 2 after CFA injection,and decreased until d 28. The expres-sion of MANF slightly increased on d 2,then remained stable,and significantly increased on d 14, and then decreased gradually. The expression of CHOP kept to rise slowly at a low level. The level of MANF in serum markedly increased on d 14,then gradually decreased, but it was still higher than the normal level on d 28. The level of CRP exhibited similar trend with MANF. Correlation analysis showed that MANF had a negative correlation with arthritis symptoms, IL-1β and TNF-α in the secondary inflammatory period of AA rats. Con-clusions Arthritis induces the expression and secre-tion of MANF,and the level of MANF is closely relat-ed to the progression and severity of arthritis.关键词
类风湿性关节炎/内质网应激/未折叠蛋白反应/MANF/分泌性蛋白/炎症因子Key words
adjuvant arthritis/endoplasmic reticulum stress/unfold protein response/MANF/secretory pro-tein/inflammatory factor分类
医药卫生引用本文复制引用
马钰泱,狄泽敏,曹晴,沈玉君,沈玉先,冯利杰..佐剂性关节炎大鼠滑膜及血清中MANF的表达及其与炎症的相关性[J].中国药理学通报,2018,34(4):537-543,7.基金项目
国家自然科学基金资助项目(No 81173074,81302755) （No 81173074,81302755）
