中国药理学通报2018，Vol.34Issue(4)：550-556,7.DOI:10.3969/j.issn.1001-1978.2018.04.022
左旋龙脑对人脐静脉内皮细胞氯通道和细胞容积的影响
Effects of L-borneol on chloride channel and cell volume in human umbilical vein endothelial cells
摘要
Abstract
Aim To study the effects of L-borneol on the chloride channel and cell volume of human umbili-cal vein endothelial cells (HUVECs). Methods Whole-cell patch-clamp technique was used to record chloride currents. The expression of ClC-3 protein was down-regulated by siRNA interference technique. The cell volume was measured by dynamic image analysis. Results 20 nmol·L-1L-borneol significantly activa-ted chloride current in HUVEC (79.59 ± 4.90) pA/pF, which could be inhibited by chloride channel blockers,NPPB and DIDS. The outward current inhib-itory rate of NPPB was (95.57 ± 2.57)%, while that of DIDS was (97.28 ± 6.36)%. The chloride current activated by L-borneol significantly decreased after the silence of ClC-3 (27.03 ± 3.89) pA/pF. Cell volume was markedly reduced by L-borneol (14.38 ± 1.58)%,which was inhibited after NPPB appliance. Conclusion L-borneol can activate ClC-3 chloride channel in HUVECs, which induces Cl- outflow then cell volume decrease.关键词
左旋龙脑/人脐静脉内皮细胞/氯通道/膜片钳技术/细胞容积/ClC-3Key words
L-borneol/human umbilical vein endo-thelial cells/chloride channel/patch-clamp tech-nique/cell volume/ClC-3分类
医药卫生引用本文复制引用
梁协稠,王立伟,陈湛如,许经逵,封洁珠,伍嘉宝,徐剑英,高宏,谭秋婵,陈丽新..左旋龙脑对人脐静脉内皮细胞氯通道和细胞容积的影响[J].中国药理学通报,2018,34(4):550-556,7.基金项目
国家自然科学基金资助项目(No 81273539,81272223) （No 81273539,81272223）
广东省科技计划项目(No 2017A050501021) （No 2017A050501021）
广东省自然科学基金资助项目(No 2016A030313495) （No 2016A030313495）
广东省科技计划项目(No 2013B051000059) （No 2013B051000059）
