中国药理学通报2018，Vol.34Issue(4)：577-583,7.DOI:10.3969/j.issn.1001-1978.2018.04.026
天麻素在甲基苯丙胺诱导SH-SY5Y细胞自噬中的作用及机制研究
Effects of gastrodin against SH-SY5Y cell autophagy induced by methamphetamine and underlying mechanisms
摘要
Abstract
Aim To investigate the effects of gastrodin on SH-SY5Y cell autophagy induced by methamphet-amine (METH) and the underlying mechanisms. Methods SY5Y cells were treated by METH with the concentration of 0.5,1.0,1.5,2.0,2.5,3.0 mmol·L-1for 24 h. The morphological changes were ob-served by microscopy,the expression of LC3-Ⅱ,Bec-lin-1,Akt,p-Akt,mTOR and p-mTOR were detected by Western blot. Gastrodin was added to the medium 1 h before METH treatment. Results The SY 5 Y cells were morphologically featured by shrinkage and den-drite disruption after exposed to METH(0~3 mmol· L-1),and autophagic vacuoles occurred in cytoplasm. The expression of LC3-Ⅱ increased over METH dose. Confocal results showed that LC3-Ⅱsignificantly in-creased in METH group as compared with control, while decreased in METH+ Gastrodin group. The ex-pression levels of LC3-Ⅱand Beclin-1 significantly in-creased (P<0.01) in METH group, p-mTOR and p-Akt decreased, and mTOR and Akt showed no signifi-cant difference as compared with control. However, the gastrodin could decrease the expression of LC3-Ⅱand Beclin-1 and increase the expression of mTOR,p-mTOR,Akt and p-Akt as compared with METH-trea-ted groups. Conclusions METH can induce SY5Y cells autophagy. The protective effect of gastrodin a-gainst METH-induced autophag may be related to gast-rodin regulation mTOR and Akt signaling pathway.关键词
甲基苯丙胺/SH-SY5Y细胞/天麻素/自噬/神经毒性/信号通路Key words
methamphetamine/SH-SY5Y cells/gast-rodin/autophagy/neurotoxicity/signaling pathway分类
医药卫生引用本文复制引用
杨根梦,李娟,洪仕君,薛凤麟,何永旺,周一卿,曾晓锋,李利华..天麻素在甲基苯丙胺诱导SH-SY5Y细胞自噬中的作用及机制研究[J].中国药理学通报,2018,34(4):577-583,7.基金项目
国家自然科学基金资助项目(No 81560303,81660310) （No 81560303,81660310）
云南省科技厅-昆明医科大学应用基础研究联合专项(No2016FE467) （No2016FE467）
云南省重大传染病疫苗研发重点实验室开放研究基金资助项目(No KF2015-01) （No KF2015-01）
昆明医科大学毒品滥用法医病理学科技创新团队(No CXTD201604) （No CXTD201604）
云南省教育厅研究生科技创新项目(No 2017YJS077) （No 2017YJS077）
