中国油料作物学报2017，Vol.39Issue(6)：771-777,7.DOI:10.7505/j.issn.1007-9084.2017.06.007
大豆组织特异启动子的克隆与功能分析
Cloning and identification of tissue specific promoter from soybean
摘要
Abstract
In this study,the soybean root specific promoter,seed specific promoter and seed coat specific promoter were cloned by homologous sequence method respectively.Their sizes were 2 500bp,1 832bp and 1 268bp.They had different expression elements including CANNTG-motifs,GATA-box,ACGT.Three plant expression vectors of these tissue specific promoters were constructed and transferred into Nicotiana tabacum NC89 by Agrobacterium-mediated method to prove GUS activity of different promoters.Soybean root,seed coat and seed specific promoter nucleotide sequence were isolated by PCR method.Then 11 root specific promoter transgenic plants,4 seed coat specific promoter transgenic plants,8 seed specific promoter transgenic plants,and 7 35S-promotertransgenic plants had been obtained.GUS activity assays indicated that GUS gene expression level in root,seed and seed coat were higher than leaf,shoot and flower.Further quantitative real time PCR results showed that the expression levels of GUS gene of root specific promoter in root were higher than those in stem,leaf,seed,seed coat and flower;the expression levels of GUS gene of seed coat specific promoter in seed coat were higher than those in root,stem,leaf,seed and flower;the expression levels of GUS gene of seed specific promoter in seed were higher than those in root,stem,leaf,seed coat and flower.But the expression levels of GUS of these three tissue specific promoters were lower than those of 35S promoter in each tissues.关键词
启动子/组织特异性/基因表达/相对表达量分析Key words
promoter/tissue specific/gene expression/relative expression analysis分类
农业科技引用本文复制引用
曹译文,宋阳,渠可心,王丕武..大豆组织特异启动子的克隆与功能分析[J].中国油料作物学报,2017,39(6):771-777,7.基金项目
大学生创新创业训练计划项目(126) （126）
科技创新基金项目(126) （126）
