摘要
Abstract
Objective To investigate the expression and clinical significance of miR-183 and Akt1 protein in endometrial carcinoma. Method From March 2014 to September 2016, 60 specimens of endometrial carcinoma and adjacent non-cancerous tissues specimens removed in first affiliated hospital of jinan university were collected, and all specimens were confirmed by pathology after operation. Cultured endometrial carcinoma cell lines Ishikawa, LipofectamineTM2000 were transfected by liposome transfection reagent, which were divided into group A (transfected with miR-183 antisense oligonucleotide specific inhibitor), group B (a specific inhibitor of the nucleotide sequence of miR-183 C, group C (transfected LipofectamineTM2000 liposome transfection reagent) and group D (blank control, notransfection). The apoptosis of four groups of endometrial carcinoma Ishikawa cells was detected by flow cytometry. The expression levels of miR-183 and Akt1 mRNA in endometrial carcinoma Ishikawa cells were detected by rea-time reverse transcription polymerase chain reaction. The expression level of Akt1 protein in endometrial carcinoma Ishikawa cells was detected by Western blotting. Result The expression of miR-183 in endometrial carcinoma was lower than that of adjacent non-cancerous tissues, and the expression of Akt1 mRNA was higher than that of adjacent non-cancerous tissues (P<0.05). The early apoptosis rate and late apoptosis rate in group A were lower than that in group B, C and D (P < 0.05), of which group B was the highest (P<0.05). The expression of miR-183 in Ishikawa cells of endometrial carcinoma of group A was lower than that of group B, C and D (P<0.05). The expression of Akt1 mRNA in Ishikawa cells of endometrial carcinoma of group A and group B was lower than that of group C and D (P< 0.05). The expression of Akt1 protein of group A was higher than that of group B, C and D (P<0.05),and group B was lower than that of group C and D (P < 0.05). Conclusion The expression of miR-183 was low in endometrial carcinoma and high expression of Akt1 protein. MiR-183 affects the proliferation and apoptosis of endometrial carcinoma Ishikawa cells by inhibiting the expression of Akt1 protein, and then affects the occurrence and development of endometrial carcinoma.关键词
miR-183/Akt1蛋白/子宫内膜癌/Ishikawa细胞Key words
MiR-183/Akt1 protein/Endometrial carcinoma/Ishikawa cell/Akt1 protein level
