中国真菌学杂志2018，Vol.13Issue(1)：4-7,4.
无绿藻PCR ITS1-ITS2基因分型法的建立及应用
The establishment and application of PCR ITS1-ITS2 genotyping method in the identification of Prototheca strains
摘要
Abstract
Objective To establish the Prototheca PCR ITS1-ITS2 genotyping method,and to generate the phylogenetic tree to study the relationship between different types of Prototheca strains.Methods The extracted DNAs of 5 clinical Prototheca isolates were used to amplify ITS1 and ITS2 region through specific primers.Then,the phylogenetic tree was established with MEGA 6.0 software and verified by SSU rDNA sequencing.Results Among the 5 strains,1 strain was identified as P.zopfii hydrocarbonea,2 strains were P.wickerhamii.Although the rest two strains couldn't be identified due to the lack of ITS sequences of P.zopfii portoricensis,they were still classified as P.zopfii and in an independent cluster which was different from P.zopfii hydrocarbonea.This result was verified by SSU rDNA sequencing.Conclusions The method of PCR ITS1-ITS2 genotyping can be used as an effective genotyping method for identification and surveillance of Prototheca infection.关键词
无绿藻/无绿藻病/基因型/鉴定/ITS区Key words
Prototheca/protothecosis/genotype/identification/ITS region分类
医药卫生引用本文复制引用
赵悦,朱巍巍,刘锦燕,孟玲宁,王珏婷,李文静,项明洁..无绿藻PCR ITS1-ITS2基因分型法的建立及应用[J].中国真菌学杂志,2018,13(1):4-7,4.基金项目
上海市黄浦区科技项目计划课题(HKW201545) （HKW201545）
上海市科委基金(15ZR1426900) （15ZR1426900）
上海市医学重点专科(ZK2012A21) （ZK2012A21）
上海市黄浦区优秀青年人才(RCPY1407) （RCPY1407）
