中国真菌学杂志2018，Vol.13Issue(1)：8-10,45,4.
CFW荧光染色法在甲真菌病诊断中的应用研究
Evaluate the accuracy of CFW fluorescent staining for the diagnosis of suspected onychomycosis
摘要
Abstract
Objective Evaluate the positive rate,accuracy and efficiency of CFW (Calcofluor white) fluorescent staining for the diagnosis of suspected onychomycosis.Methods Outpatients suspected to have onychomycosis were recruited.Each patient was subjected to three methods (CFW fluorescent staining,KOH microscopy and fungal culture) together to confirm the diagnosis.Then we compared the positive rates and the statistically significant differences across the three methods.Moreover,the images under KOH and CFW were also compared.Results One hundred patients with 100 affected nails were recruited.Among them,the positive rates of the CFW,KOH,and culture were 64 ％,63 ％,and 31％,respectively.According to the McNemar test,CFW had a higher percentage of positive rate than culture testing (P＜0.01),but it was not statistically significant different from KOH (P＞0.05).Compared with KOH,the preparation and examination time were faster and easier,and the fungal structures under CFW were clearer,more obvious,and more easily detected.Conclusions CFW fluorescent staining was a simple,fast,convenient,accurate,and time-efficient method.It could potentially increase the accuracy and efficiency of the diagnosis of onychomycosis and help to avoid misdiagnosis.关键词
甲真菌病/CFW荧光染色/KOH溶液湿片法/真菌培养Key words
onychomycosis/CFW fluorescent staining/KOH microscopy/fungal culture分类
医药卫生引用本文复制引用
岳学苹,石秀艳,陈伟,万喆,李若瑜,王爱平,李清..CFW荧光染色法在甲真菌病诊断中的应用研究[J].中国真菌学杂志,2018,13(1):8-10,45,4.基金项目
北京市自然科学基金(7164255) （7164255）
