浙江医学2018，Vol.40Issue(3)：224-230,7.DOI:10.12056/j.issn.1006-2785.2018.40.3.2017-418
浸润性肺腺癌组织上调表达基因的筛选及验证
Screening and verification of up-regulated genes in invasive lung adenocarcinoma
摘要
Abstract
Objective To screen and verify the up-regulated genes in invasive lung adenocarcinoma.Methods Tissue samples from 6 cases of invasive lung adenocarcinoma confirmed by surgery and pathology were collected.Gene expression profiling chips for cancer tissue and adjacent tissue were made.The chips data were analyzed with Gene Ontology,Pathway Analysis,Marker Analysis,Gene Network Relationship Analysis.Up-regulated genes in invasive lung adenocarcinoma were screened,with the data base of The Lung Cancer and mRNA information related to lung cancer.The expression of up-regulated genes in cancer and pericancerous tissue samples of 97 patients with invasive lung adenocarcinoma were detected by RT-PCR and compared between cancer and pericancerous tissues.Results Fourteen up-regulated expression genes in invasive lung adenocarcinoma were screened by the microarray and the database.The up-regulated expression genes were SPINK1,ITGA11,TOX3,SPP1,MCM10,MMP12,COL11A1,SIX1,FOXA1,CLIC6,TMEM45B,GDF15,CEACAM1 and ESR1.Compared with paracancerous tissues,the mRNA expressions of SPINK1,TOX3,MMP12,CLIC6 and TMEM45B were up-regulated in cancer tissues(P＜0.01or 0.05).There was no significant difference in the mRNA expressions of ITGA11,SPP1,MCM10,COL11A1,SIX1,FOXA1,TMEM45B,GDF15,CEACAM1 and ESR1 between two groups(P ＞0.05).Conclusion The expressions of SPINK1,TOX3,MMP12,CLIC6 and TMEM45B mRNA are up-regulated in invasive lung adenocarcinoma,the value of these gene need to be further explored.关键词
浸润性肺腺癌/基因芯片/差异表达基因/分子标记物Key words
Invasive lung adenocarcinoma/Gene microarray/Differentially expressed genes/Molecular markers引用本文复制引用
刘超武,续力云,陈军,乐涵波..浸润性肺腺癌组织上调表达基因的筛选及验证[J].浙江医学,2018,40(3):224-230,7.基金项目
浙江省科技厅分析测试科技计划项目(2015C37024) （2015C37024）
浙江省医药卫生科技计划一般项目(2015KYB411) （2015KYB411）
舟山市公益类科技项目(2014C31065) （2014C31065）
