浙江医学2018，Vol.40Issue(3)：231-234,239,5.DOI:10.12056/j.issn.1006-2785.2017.39.22.2016-853
si-RNA介导FGFR1基因沉默对胃癌细胞生长及凋亡的影响
Effect of siRNA-mediated FGFR1 gene silencing on growth and apoptosis of gastric cancer cell line SGC-7901
许超超 1金嵘 2颜小丹 1叶丽萍1
作者信息
- 1. 317000 台州医院消化内科
- 2. 温州医科大学附属第一医院流行病学教研室
- 折叠
摘要
Abstract
Objective To investigate the effect of siRNA-induced FGFR1 gene down-regulation on proliferation and apoptosis of gastric cancer SGC-7901 cells.Methods siRNA-FGFR1 and the negative-control(NC)-siRNA were designed and synthesized,then transfected into SGC-7901 cells.The expression levels of FGFR1 protein was detected by Western blotting analysis.The MTT assay and cell clonogenic assay were used to investigate the inhibitory effect of siRNA-FGFR1 on the viability of gastric cancer SGC-7901 cells.The apoptosis of SGC-7901 cells was detected by flow cytometry with Hoechst staining.Results The expression of FGFR1 in SGC-7901 cells was higher than that in human normal gastric mucosa epithelial GES-1 cells (P＜0.05),and the expression level of FGFR1 in SGC-7901 cells after transfection with siRNA-FGFR1 was down-regulated (P＜0.01).The MTT and clonogenic assay showed that the growth of SGC-7901 cells was inhibited after transfecton with siRNA-FGFR1 in comparison to that in NC-siRNA and mock groups.Flow cytometry and Hoechst staining showed that the apoptotic rate was significantly increased in siRNA-FGFR1 transfected cells compared to control cells.Conclusion siRNA-FGFR1 can significantly inhibit FGFR1expression,suppress the growth and induce apoptosis in FGFR1-overexpressing gastric cancer SGC-7901 cells.关键词
胃癌/增殖/凋亡/成纤维细胞生长因子受体1Key words
Gastric cancer (GC)/Proliferation/Apoptosis/Fibroblast growth factor receptor 1 (FGFR1)引用本文复制引用
许超超,金嵘,颜小丹,叶丽萍..si-RNA介导FGFR1基因沉默对胃癌细胞生长及凋亡的影响[J].浙江医学,2018,40(3):231-234,239,5.
