浙江医学2018，Vol.40Issue(3)：248-251,4.DOI:10.12056/j.issn.1006-2785.2017.40.3.2016-1089
重症社区获得性肺炎患者外周血单核细胞Toll样受体4的表达及临床意义
Expression of Toll-like receptor 4 in mononuclear cells of patients with severe community-acquired pneumonia and its clinical significance
摘要
Abstract
Objective To investigate the expression of toll like receptors 4 (TLR4) in peripheral blood mononuclear cells of patients with severe community-acquired pneumonia (SCAP),and explore its clinical significance.Methods Fifty patients with severe community-acquired pneumonia (SCAP group) admitted in Department of Respiration and intensive care unit from 2014 January to 2015 January and 50 healthy subjects were enrolled in the study (control group).Peripheral blood mononuclear cells (PBMCs) were separated and mRNA was extracted,the expression of TLR4 mRNA was detected with real-time quantitative PCR (qRT-PCR) in both groups.Enzyme-linked immunosorbent assay (ELISA) was used to detect tumor necrosis factor (TNF-α),IL-6,IL-8 and other related inflammatory factors in serum of two groups.After lipopolysaccharide (LPS) was added into peripheral venous blood,TLR4 mRNA and related inflammatory factors was detected.The correlation between TLR4 mRNA expression and pneumonia severity index (PSI) score in SCAP patients was analyzed.Results The relative expression of TLR4 mRNA and the levels of inflammatory cytokines in SCAP group were significantly higher than those in control group(P＜0.05).After adding LPS,the levels of TLR4 mRNA expression and inflammatory factors were increased in both groups,but the levels were increased less markedly in SCAP group.The expression of TLR4 mRNA was positively correlated with the score of PSI in SCAP group.Conclusion The TLR4 signal pathway is involved in the development of SCAP.Blocking TLR4 receptor may inhibit the release of inflammatory cytokines and reduce lung tissue damage,which may provide a new target for clinical treatment of SCAP.关键词
肺炎/Toll样受体4/单核细胞Key words
Pneumonia/Toll-like receptor 4/Monocytes引用本文复制引用
李俏俏,李隆祥,罗建华,王晨,潘君素..重症社区获得性肺炎患者外周血单核细胞Toll样受体4的表达及临床意义[J].浙江医学,2018,40(3):248-251,4.基金项目
浙江省中医药科技计划(2017ZB098) （2017ZB098）
台州市椒江区科技计划项目(132057) （132057）
