浙江医学2018，Vol.40Issue(4)：325-328,332,5.DOI:10.12056/j.issn.1006-2785.2017.40.4.2017-2366
Cocl2诱发急性化学缺氧对非酒精性脂肪肝小鼠的影响
The effect of Cocl2-induced acute chemical hypoxia on nonalcoholic fatty liver mice
摘要
Abstract
Objective To investigate the effect of Cocl2-induced acute chemical hypoxia on FGF21 expression in nonalcoholic fatty liver mice.Methods Thirty male C57BL/6 mice were randomly divided into three groups with 10 in each group:normal diet group (control),high fat diet group (HFD),high fat diet plus cobalt chloride hypoxia group (HFD+Cocl2).The animals were sacrificed after 8 weeks of feeding and serum samples and liver tissue were collected.The levels of fasting blood glucose,TG,TC and ALT were measured by automatic biochemical analyzer.The content of FGF21 in serum was detected by ELISA.The morphological changes of liver tissue were observed with HE staining and three colors staining.The expression of TIMP-1,HIF-1a and FGF21 mRNA was detected by Q-PCR,and the expression of FGF21,JNK and XBP-1 protein was detected by Western blot.Results Compared with control group,the serum levels of fasting blood glucose,TC,ALT and FGF21 in HFD group and HFD+Cocl2 group were significantly increased (P＜0.05),the formation of intrahepatic fibrosis in HFD group and HFD+Cocl2 group was aggravated,the expressions of TIMP-1,FGF21 mRNA and FGF21,p-JNK,XBP-1 protein in liver tissue of HFD group and HFD+Cocl2 group were up-regulated (P＜0.05).Compared with the HFD group,the levels of TG,ALT and FGF21 in the serum of HFD+Cocl2 group were further increased (P＜0.05).The formation of intrahepatic fibrosis in HFD+Cocl2 group was more aggravated.The expression of TIMP-1,FGF21 mRNA and FGF21,p-JNK and XBP-1 protein in the liver of HFD+Cocl2 group were increased more markedly.Conclusion Acute chemical hypoxia aggravates liver injury in nonalcoholic fatty liver mice and up-regulates the expression of FGF21.The endoplasmic reticulum stress might be the pathways of hypoxia-induced lipid metabolism disorder.关键词
缺氧/非酒精性脂肪肝/成纤维细胞生长因子21/内质网应激Key words
Hypoxia/Nonalcoholic fatty liver/Fibroblast growth factor 21/Endoplasmic reticulum stress引用本文复制引用
张学松,张谢,叶桦,宋毓飞..Cocl2诱发急性化学缺氧对非酒精性脂肪肝小鼠的影响[J].浙江医学,2018,40(4):325-328,332,5.基金项目
国家自然基金项目(81400617) （81400617）
浙江省自然基金项目(LQ14H030001) （LQ14H030001）
浙江省医药卫生科技项目(2016KYB271) （2016KYB271）
宁波市自然基金项目(2015A610182) （2015A610182）
宁波市自然基金项目(2016A610194) （2016A610194）
