浙江医学2018，Vol.40Issue(4)：339-341,364,4.DOI:10.12056/j.issn.1006-2785.2017.40.4.2017-413
miR-34b促进关节软骨细胞基质退变的分子机制研究
Molecular mechanism of mirR-34b in promoting degeneration of articular cartilage matrix
摘要
Abstract
Objective To explore the molecular mechanism of miR-34b in promoting the degeneration of articular cartilage matrix.Methods Cartilage specimens from 10 osteoarthritis patients (OA group) and 7 traumatic amputees (control group) were collected.The levels of miR-34b in cartilage specimens was measured by using real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR).Bioinformatics software Targetscan and luciferase assay were used to test whether Smad3 was the target gene of miR-34b.The cultured SW1353 cells were transfected with equivalent liposomes (groups A),scrambled oligonucleotides(group B),miR-34b mimic(group C) and miR-34b inhibitor(group D),respectively.The expression of miR-34b,Smad3,type Ⅱ collagen and matrix metalloproteinase-3 (MMP-13) were detected by RT-qPCR.Results Expression level of miR-34b was significantly higher in OA cartilages than that in normal cartilages (P＜0.05).The expression of miR-34b was significantly up-regulated in group C(P＜0.05) and inhibited in group D(P＜0.05),compared to group A;while there was no significant difference between group A and group B (P ＞0.05).The results of bioinformatics software Targetscan and luciferase assay showed that Smad3 was one of the target genes of miR-34b.After transfection of miR-34b mimic and miR-34b inhibitor,the mRNA expression level of Smad3 decreased in group C (P＜0.05) and increased significantly in group D (P＜0.05),compared to group A.While there was no significant difference between group A and group B (P ＞0.05).After transfection,the mRNA expression level of type Ⅱ collagen decreased in group C (P＜0.05),and increased in group D (P＜0.05),compared with group A.The expression of MMP-13 increased in group C (P＜0.05),and decreased in group D (P＜0.05),compared to group A.Conclusion MiR-34b may promote the degeneration of human articular cartilage matrix in osteoarthritis by targeting Smad3.关键词
微小RNA-34b/Smad3/骨关节炎/软骨细胞基质Key words
miR-34b/Smad3/Osteoarthritis/Cartilage extracellular matrix引用本文复制引用
王丹丹,陈刚,叶俏,鲍轶,官俏兵..miR-34b促进关节软骨细胞基质退变的分子机制研究[J].浙江医学,2018,40(4):339-341,364,4.基金项目
嘉兴市科技局计划项目(2013AY21058) （2013AY21058）
