中医药学报2017，Vol.45Issue(6)：24-27,4.
不同生长期一枝蒿中3种化学成分的含量测定
Contents Determination of Three Chemical Constituents in Artemisia Rupestris L.at Different Growth Stages
摘要
Abstract
Objective:To establish the HPLC-DAD method to determinate the content of Rupestonic acid,Chlorogenic acid,and Luteolin in Artemisia Rupestris L.at different growth stages.Methods:The chromatographic column was Agilent ZORBAX SB-C18(4.6 mm× 250 mm,5 μm) with 0.5％ glacial acetic acid(A)-acetonitrile(B),gradient elution(0 ～10 min,10.5％ ～14.5％ B;12～23 min,22％～26％;29～46 min,27.5％～28.5％ B).The detection wave of Rupestonic acid was about 250 nm,and Chlorogenic acid and Luteolin was about 338nm.The column temperature was 35 ℃,and the flow rate was 1 mL/min.Results:The linearity ranges of Rupestonic acid,Chlorogenic acid and Luteolin were 0.306 3 ～4.90 μg(r =0.999 9),0.1338 ～2.14 μg(r =0.999 4),0.023 9 ～0.143 μg(r =0.999 1),which showed that there was a good linear relationship.The average recoveries were 95.7％ (RSD =0.75％),103.2％ (RSD =1.77％),and 100.7％ (RSD =2.64％) respectively.Conclusion:This method is simple,reliable and accurate.It can effectively monitor the changes of the three chemical components in Artemisia Rupestris L.at different growth stages,and provide the theoretical basis for determining the reasonable harvest time.关键词
一枝蒿/一枝蒿酮酸/绿原酸/木犀草素Key words
Artemisia Rupestris L./Rupestonic acid/Chlorogenic acid/Luteolin分类
医药卫生引用本文复制引用
兰冬冬,朱国强,郭雄飞,田树革..不同生长期一枝蒿中3种化学成分的含量测定[J].中医药学报,2017,45(6):24-27,4.基金项目
第四次全国中药资源普查(试点)新疆中药民族药资源普查专项资助(2015-2016) （试点）
