中医药学报2017，Vol.45Issue(6)：32-36,5.
二仙补肾益精解毒方对弱精子症大鼠模型氧化应激损伤的保护机制研究
Protective Mechanism of Erxian Bushen Yijing Jiedu Formula for Asthenospermia Rats with Oxidative Stress Injury
摘要
Abstract
Objective:To observe the protective effect of Erxian Bushen Yijing Jiedu formula improving sperm motility in asthenospermia rats with oxidative stress injury induced by Ornidazole,and to explore its possible mechanism.Methods:72 rats were randomly divided into the blank control group,the model group,the Levocamitine group,and low-dose medication group,middle-dose medication group,and high-dose medication group,the medication groups were treated with Erxian Bushen Yijing Jiedu formula.After four-week treatment,sperm count and sperm motility were evaluated,pathological changes were observed in seminiferous tubules of testicles,and contents of MDA,SOD and GSH-Px in testicles and epididymal tissues were determinated by spectrophotometry.Results:Grade B sperm productivity showed an improved tendency in the three medication groups and in the Levocarnitine group compared to the model group;the content of MDA was decreased,and in the contents of SOD and GSH were increased significantly in the three medication groups and in the Levocarnitine group compared to the model group;Pathological studies showed that a large number of spermatogenic cells were found in the seminiferous tubules in the middle-dose medication group and the Levocarnitine group,which were similar to those in the blank control group.Conclusion:Erxian Bushen Yijing Jiedu formula can improve sperm motility and the resistance of generative cells to ROS.关键词
弱精子症/氧化应激/精子活动力Key words
Asthenospermia/Oxidative stress/Sperm motility分类
医药卫生引用本文复制引用
刘绍明,郭军,张岳阳,时建华,丁红阳,张修举,陈小均..二仙补肾益精解毒方对弱精子症大鼠模型氧化应激损伤的保护机制研究[J].中医药学报,2017,45(6):32-36,5.基金项目
国家自然科学基金面上项目(81473527) （81473527）
