中医药学报2017，Vol.45Issue(6)：57-61,5.
黄芩素延缓大鼠心肌衰老的作用及机制
Myocardial Protective Effect of Baicalein on Aging Rats Induced by D-galactose
李爽 1刘璐 1胡宝荣 1张婧 1宋永熙 1海鑫1
作者信息
- 1. 哈尔滨医科大学附属第一医院药学部,黑龙江哈尔滨150001
- 折叠
摘要
Abstract
Objective:To study the protective effect of baicalein on the aging model rats and explore the underlying mechanism.Methods:75 rats were randomly divided into the blank control group,the model group,high-dose baicalein group(200mg/kg),medium-dose baicalein group (100 mg/kg),and low-dose baicalein group (50 mg/kg),with 15 rats in each group.The aging models were established by the injection of D-galactose solution(150 mg/kg) for 30d,whereas the blank control group was injected saline(10 mL/kg).Different concentrations of baicalein were applied to the rats in baicalein groups for 30d after modeling,at the same time,same volume of saline was given to the blank control group and the model group.Cardiac function was evaluated with Echocardiography,and SOD,GSH-Px and MDA were tested in the myocardium.SA-β-gal activity and expressions of p21cip1/Waf1 and p53 were examined.Results:Compared to blank control group,in the model group,the cardiac function of aging rats was declined(P ＜0.01);the activity of SOD and GSH-Px in the myocardium were decreased significantly(P ＜ 0.01);SA-β-gal-positive cells and MDA contents in the myocardium were increased significantly (P ＜ 0.01);expressions of p53 and p21 cip1/Waf1 were also increased;Compared to the model group,the cardiac function in the baicalein groups was improved;the activities of SOD and GSH-Px in myocardium were increased significantly,with decreased MDA SA-β-gal-positive cells in the baicalein groups(P ＜0.01);the expressions of p53 and p21cip1/Waf1 were also significantly decreased in baicalein groups.The anti-aging effect of baicalein was dose dependent,the high-dose group improved more than the other two groups.Conclusion:Baicalein has obvious protective effect on myocardial cells in aging rats induced by D-galactose,the mechanism may be related to the decreased oxidative stress level and inhibiting its downstream p53/p21Cip1/Waf1 signaling pathway,improving heart function in aging rats.关键词
黄芩素/抗衰老/大鼠/氧化损伤/心脏Key words
Baicalein/Anti-aging/Rats/Oxidative stress/Heart分类
医药卫生引用本文复制引用
李爽,刘璐,胡宝荣,张婧,宋永熙,海鑫..黄芩素延缓大鼠心肌衰老的作用及机制[J].中医药学报,2017,45(6):57-61,5.
