中医药学报2018，Vol.46Issue(2)：20-24,5.DOI:10.19664/j.cnki.1002-2392.180040
薏苡仁与花旗泽仁给药后9-HODE在大鼠血浆中的药动学比较
Pharmacokinetics Comparison of 9-HODE after the Administration of HQZR and Yiyiren
摘要
Abstract
Objective:To compare the pharmacokinetics 9-hydroxy-(10E,12E)-octadecadienoic acid(9-HODE) from single Yiyiren(YYR)and compound Huaqizeren(HQZR)in the serum of rats by HPLC -MS/MS method. Methods:Male SD rats were randomly divided into HQZR group and YYR group.Plasma concentration of 9-HODE was determined by HPLC -MS/MS method at different time points.Results:The HPLC-MS/MS analysis method es-tablished in this study had good precision and method specificity.The recovery, reproducibility and stability of each component were in line with the requirements of biological samples for the detection of plasma 9-HODE.Cmax of 9-HODE was 802.9 ±88.64 μg/L;Tmax was 0.5 h;t1/2was 7.737 ±3.309 h,and AUC0-twas 1820 ±154.2 μg/L·h;MRT0-twas 5.862 ±1.304 h;CL was 0.190 5 ±0.013 22 L/h/kg.The results of this experiment showed that CL was significantly decreased in HQZR group compared to that in YYR group(P<0.05).There were no significant difference between the two groups in terms of AUC,MRT0-t,and t1/2(P>0.05).Conclusion:HQZR dose not have a significant impact on the blood concentration of 9-HODE absorption, it can reduces CL and prolong the action duration of 9-HODE.关键词
花旗泽仁/薏苡仁/9-HODE/比较药代动力学/HPLC-MS／MSKey words
Huaqizeren（HQZR）/Yiyiren（Fructus Alpniae Oxyphyllae）/9-HODE/Comparative pharmacokinetics/HPLC-MS／MS分类
医药卫生引用本文复制引用
孙雨,韩东卫,朱蕾,葛鹏玲..薏苡仁与花旗泽仁给药后9-HODE在大鼠血浆中的药动学比较[J].中医药学报,2018,46(2):20-24,5.基金项目
国家科技重大专项(2012ZX09103201-018) （2012ZX09103201-018）
国家自然科学基金项目(81273650) （81273650）
黑龙江省自然科学基金项目(LC2011C03) （LC2011C03）
黑龙江中医药大学“优秀创新人才支持计划”(2012RCD19) （2012RCD19）
黑龙江省博士后科研启动基金项目(LBH-Q15136) （LBH-Q15136）
哈尔滨市科技创新人才研究专项资金项目(2016RAXXJ100) （2016RAXXJ100）
黑龙江中医药大学科研基金项目(201515) （201515）
黑龙江省中医药科研项目(ZHY16-093) （ZHY16-093）
