中医药学报2018，Vol.46Issue(2)：25-29,5.DOI:10.19664/j.cnki.1002-2392.180041
HPLC-ELSD法同时测定商陆中四种皂苷的含量
Simultaneous Determination of Four Triterpenes in Radix Phytolaccae by HPLC-ELSD
摘要
Abstract
To develop the HPLC-ELSD method for simultaneous determination of four triterpenes,including esculento-side A,esculentoside B,esculentoside C,and esculentoside H.Methods: The Diamonsil C18 column(250 mm×4.6 mm,5 μm)was used.The mobile phase was methanol(A)and 0.1%acetic acid(B)with a gradient elution(0-20 min,50%A-90%A), the flow rate was 1.0 mL·min-1.HPLC-EISD was applied to determine the content, the column temperature was 30℃,gas flow rate was 2.0 L· min -1, and drift tube temperature was 50℃.Results: The calibration curves of esculentoside A,esculentoside B,esculentoside C and esculentoside H showed good liner relation-ship between logarithm of the peak area and logarithm of the content of each compound over 0.719-8.628,0.376-4.518,0.258-3.102,and 0.503-6.036 μg respectively(r=0.9992-0.9996).The average recoveries(n=6) were in the ranges of 99.52%-100.17%and RSD was in the ranges of 1.83%-2.34%respectively.The contents in 30 batches of samples of Radix Phytolaccae were 1.53-4.58 mg· g-1for esculentoside A,0.16-1.40 mg· g -1for esculentoside B,0.10-0.61 mg· g-1for esculentoside C, and 0.13-4.98 mg· g-1for esculentoside H.Conclu-sion:The method is precise and repeatable,which can be used to determine the contents of four triterpenoid saponins in Radix Phytolaccae.关键词
商陆/高效液相色谱-蒸发光散射检测/商陆皂苷A/商陆皂苷B/商陆皂苷C/商陆皂苷H/含量测定Key words
Shanglu（Radix Phytolaccae）/HPLC -ELSD/Esculentoside A/Esculentoside B/Esculentoside C/Esculentoside H/Content determination分类
医药卫生引用本文复制引用
姜希红,王知斌,王秋红,匡海学..HPLC-ELSD法同时测定商陆中四种皂苷的含量[J].中医药学报,2018,46(2):25-29,5.基金项目
国家重点基础研究发展计划(973计划)项目(2013CB531801) （973计划）
