安徽农业大学学报2018，Vol.45Issue(1)：6-14,9.DOI:10.13610/j.cnki.1672-352x.20180302.021
茶氨酸衍生物TClC脂质体对雌激素受体阳性的乳腺癌细胞生长和迁移的抑制作用
Inhibitory effects of theanine derivative TClC liposome on the growth and migrations of ER-α-positive human breast cancer cells
摘要
Abstract
The purpose of this study was to investigate the inhibitory effects of a theanine derivative TC1C-prepared liposome (TClC-L) on the growth and migrations of human breast cancer MCF-7 cells and its potential mechanism.The assays of MTT and transwell chamber were used to explore the effects of TC1C-L on the MCF-7 cell grown and migration,respectively.The flow cytometry (FCM) and Western blot were applied to analyzing the effects of TC1C-L on the apoptosis and related protein expressions in MCF-7 cells,respectively.The experimental results indicated that TC1C-L inhibited the cell growth and migration and promoted the apoptosis of human breast cancer cells.TC1C-L significantly down-regulated the protein expression and/or phosphorylation of Bcl-2,p-VEGFR2,Met,ER-α,Cyclin D 1,Akt and NF-κB,while it up-regulated the protein expression of Bax,caspase-3,p53,and p21 in MCF-7 cells.The possible molecular mechanism of the TC1C-L action against MCF-7 cells might be connected with the suppression of the signal pathway of VEGFR/Met/ER-α-Akt/NF-κB.TClC-L has a broad application prospect in treating estrogen receptor-positive human breast cancer.关键词
茶氯香酰胺脂质体/雌激素受体阳性的人乳腺癌/生长和迁移/细胞凋亡/抑制作用机制Key words
TClC-L/estrogen receptor-positive breast cancer/growth and migration/apoptosis/mechanisms of inhibition分类
农业科技引用本文复制引用
杨美玲,吴犇昊,张国营,刘真真,刘辉,朱荣芹,李筝,闫春燕,盖静,郑晓慧,孙考祥..茶氨酸衍生物TClC脂质体对雌激素受体阳性的乳腺癌细胞生长和迁移的抑制作用[J].安徽农业大学学报,2018,45(1):6-14,9.基金项目
国家科技部十二五“863”项目(2012AA020206),十三五国家重点研发计划项目(2017YFB0702600)和山东省自然科学基金项目(ZR2015HM004)共同资助. （2012AA020206）
