安徽农业大学学报2018，Vol.45Issue(1)：15-24,10.DOI:10.13610/j.cnki.1672-352x.20180302.023
茶氨酸衍生物茶溴香酰胺脂质体对人肺癌细胞生长和迁移的抑制作用
Inhibitory effects of theanine derivative TBrC-prepared liposome on the growth and migration of human lung cancer cells
摘要
Abstract
This study is to investigate the inhibitory effects of theanine derivative TBrC-prepared liposome (TBrC-L) on the growth and migration of human non-small-cell lung cancer (NSCLC) A549 cells and its molecular mechanism.The MTT assay and flow cytometry were used to detect the effects of different concentrations of TBrC-L on the growth and apoptosis of human lung cancer A549 cells,respectively.The transwell chamber assay and westem blot were applied to determining the effects of TBrC-L on the cancer cell migration and related protein expressions,respectively.The results showed that TBrC-L significantly inhibited the cancer cell growth and migration and induced apoptosis in human lung cancer A549 cells.The suppression of the cancer cell growth and migration as well as the induction of apoptosis may be the important anticancer activities of TBrC-L against the human lung cancer A549 cells.The molecular mechanism of TBrC-L action may be involved in restraining the EGFR-,VEGFR1-,VEGFR2-and Met-mediated signaling transduction pathways of Akt and NF-κB in the human cancer cells.The results suggested that TBrC-L may have the potential to be used in clinical treatment and/or adjuvant treatment of human non-small-cell lung cancer.关键词
茶溴香酰胺脂质体/人肺癌/生长和迁移/抑制作用机制Key words
TBrC-L/human lung cancer/growth and migration/mechanisms of inhibition分类
农业科技引用本文复制引用
闫春燕,吴犇昊,张国营,刘辉,朱荣芹,刘真真,李筝,孙考祥,杨美玲,盖静,郑晓慧..茶氨酸衍生物茶溴香酰胺脂质体对人肺癌细胞生长和迁移的抑制作用[J].安徽农业大学学报,2018,45(1):15-24,10.基金项目
国家科技部十二五“863”项目(2012AA020206),十三五国家重点研发计划项目(2017YFB0702600)和山东省自然科学基金项目(ZR2015HM004)共同资助. （2012AA020206）
