安徽农业大学学报2018，Vol.45Issue(1)：187-194,8.DOI:10.13610/j.cnki.1672-352x.20180302.005
辣椒RWP-RK转录因子分析
Analysis of pepper RWP-RK transcription factors
摘要
Abstract
The RWP-RK gene family is a plant-specific transcription factor family.RWP-RK transcription factors play central roles in regulation of plant nitrogen signal transduction and gametophyte development.Using the genomic sequences of "Capsicum annuum L.Zunla-1",an important pepper cultivar in China and its ancestor Mexican pepper "Chiltepin",we identified the genes containing the RWP-RK domain in these two pepper genomes.We also carried out phylogenetic,chromosome localization,conserved sequences and possible function analysis.In the genome of Capsicum annuum L.Zunla-1,CaZunla01g000097,CaZunla01g004485 and CaZunla00g000405 were homologous to the essential regulatory factor (AtNLP7) controlling the nitrogen metabolism in Arabidopsis thaliana.Capana03g001990 and Capana03g000787 were homologous to Arabidopsis thaliana egg formation related transcription factors AtRKD1,AtRKD2 and AtRKD4.These genes maintain a high degree of conservation between the cultivar Zunla-1 and its wild ancestor "Chiltepin".In addition,there is a unique RWP-RK transcription factor subfamily in the pepper genomes.This subfamily protein contains unique motifs that are not existed in the Arabidopsis RWP-RK transcription factors.There is also a RWP-RK transcription factor unique in Capsicum annuum L.Zunla-1,but not in the wild ancestor "Chiltepin".In this study,we identified RWP-RK transcription factors that are highly similar or different to Arabidopsis RWP-RK transcription factors.These identified factors showed significant differences between the wild and cultivars of pepper.The results provide important information for pepper RWP-RK genes cloning and functional analysis.Functional studies on these genes will greatly promote the study of the molecular mechanism of peppers in response to nitrogen deficiency and pepper gametophyte development.关键词
辣椒/RWP-RK转录因子/结构域/亚家族/氮元素Key words
Capsicumspp./RWP-RK transcription factor/protein domain/subfamily/nitrogen分类
生物科学引用本文复制引用
李菲,龚记熠,何小红,张习敏,乙引..辣椒RWP-RK转录因子分析[J].安徽农业大学学报,2018,45(1):187-194,8.基金项目
贵州省科学技术基金(2017GZ66249)资助. （2017GZ66249）
