渔业科学进展2018，Vol.39Issue(2)：42-48,7.DOI:10.19663/j.issn2095-9869.20170102001
三株异养硝化-好氧反硝化细菌对圆斑星鲽养殖水质的净化效果
Purifying Effect of Three Heterotrophic Nitrification-Aerobic Denitrification Bacteria Strains on the Farming Water of Verasper variegates
摘要
Abstract
High nitrogen concentration in intensive aquaculture systems has recently become a serious problem to impact the survival of farmed animals and the marine environment.The newly found heterotrophic nitrification-aerobic denitrification bacteria showed several advantages in removing the nitrogen in the waster waters.To investigate the purifying effect of three heterotrophic nitrificationaerobic denitrification bacteria strains on waste water of Verasper variegates farming,240 Verasper variegates (98+6) g were divided into 8 groups randomly.Simultaneously,the various combinations of Bacillus hwajinpoensis (SLWX2),Halomonas alkaliphila (X3),and Alteromonas macleodii (SLNX2) were inoculated into the culture tanks respectively.Different inorganic nitrogen and organic matter of the various combinations were determined.The results showed that the concentrations of ammonia-nitrogen,nitrite-nitrogen,nitrate-nitrogen,total nitrogen,and chemical oxygen demand in the control group during the whole experiment were from 0.21 mg/L to 15.94 mg/L,0.08 mg/L to 5.68 mg/L,1.10 mg/L to 7.05 mg/L,1.74 mg/L to 38.86 mg/L,and 1.19 mg/L to 22.87 mg/L,respectively.The concentrations of the indices in the trial groups were significantly lower than those of the control group.Among the trial groups,the group SLWX2+X3+SLNX2 showed the best purifying effect with the concentrations of ammonia-nitrogen,nitrite-nitrogen,nitrate-nitrogen,total nitrogen and chemical oxygen demand 68.55％,48.36％,58.38％,40.02％,and 27.47％ lower than those in the control group,respectively.The group SLWX2+X3 showed the second best purifying effect.All trial groups had significantly lower fish death at the 21st day compared with the control group.The results indicated that these three heterotrophic nitrification-aerobic denitrification bacteria strains can effectively improve the water quality through nitrification and denitrification without adding carbon sources,and have no toxic or morbidity effect on Verasper variegatus.关键词
异养硝化-好氧反硝化细菌/净化效果/细菌组合/圆斑星鲽Key words
Heterotrophic nitrification-aerobic denitrification bacteria strains/Purification effect/Bacteria combinations/Verasper variegates分类
资源环境引用本文复制引用
康传磊,李秋芬,张艳,陈世波,王越..三株异养硝化-好氧反硝化细菌对圆斑星鲽养殖水质的净化效果[J].渔业科学进展,2018,39(2):42-48,7.基金项目
海洋公益性行业科研专项(201305043)和国家自然科学基金项目(31170113)共同资助[This work was supported by the National Marine Public Welfare Research Project (201305043),and National Natural Science Foundation of China (31170113)] （201305043）
