渔业科学进展2018，Vol.39Issue(2)：59-66,8.DOI:10.19663/j.issn2095-9869.20161229001
短期温度胁迫对驼背鲈(♀)×鞍带石斑鱼((♂))杂交子代幼鱼抗氧化及消化酶活性的影响
Effects of Short-Term Temperature Stress on Antioxidant and Digestive Enzymes of Hybrid Progeny (Cromileptes altivelis Valenciennes ♀ × Epinephelus lanceolatus (♂))
摘要
Abstract
The effects of variable temperature acute change (21℃,24℃,28℃,32℃ and 35℃) and temperature gradient (rising 1 ℃ every day) on the antioxidant and digestive physiology in hybrid progeny (Mussaurus spot) of Cromileptes altivelis Valenciennes♀× Epinephelus lanceolatus(♂) were studied.The results showed that feed intake and digestive enzyme activity in gradual rising temperature group were higher than those in temperature acute change groups.The temperature acute change groups:the feed intake and digestive enzyme activity were gradually increased from 21 ℃ to 32 ℃,but decreased in 35℃ group.The serum SOD activities were increased significantly in most temperature mutations groups except 35℃ group at the 7th day.The liver SOD activities had no significant difference before and after treatment in 21 ℃,24℃ and 28℃ groups,but decreased in 32℃ group and increased in 35℃ group.The activities of catalase enzyme (CAT) in serum and liver had increasing trends in 21 ℃,24℃ and 28℃ groups,but had decreasing trends in 32℃ and 35℃ groups.The serum MDA content increased at first and then decreased in 32℃ group and the amplitude of variation was significantly larger than other groups with gradually decreased content of MDA.The liver MDA content increased at first and then decreased in all groups.The gradual rising temperature group:The serum SOD and CAT activities increased at first and then decreased.The liver SOD activity had no significant change at the 3rd day but significantly increased at the 7th day,while the liver CAT activity decreased significantly at both the 3rd and 7th day.The MDA content in both liver and serum were gradually declined.The temperature acute change groups:the intestinal tract pepsin and lipase activities were similar in 21℃ and 24℃ groups,which decreased at first and then increased.The pepsin activity gradually increased in other groups,while the lipase activity increased at first and then decreased.The amylase activity maintained at a lower level without obvious change.The activity of pepsin and lipase increased significantly in the gradual rising temperature group,while there was no significant change in the amylase activity.In summary,temperature gradual rising can promote the feed intake and antioxidant ability of juvenile Mussaurus spot,while the temperature acute change can make significant effect on antioxidant system and may cause liver damage overtime.So temperature acute change should be limited to reduce the adverse stress response during the feeding time,and temperature gradual rising can be used to achieve the required conditions.关键词
驼背鲈×鞍带石斑鱼杂交子代/温度胁迫/抗氧化性/消化酶Key words
Hybrid progeny (Cromileptes altivelis Valenciennes ♀ × Epinephelus lanceolatus (♂))/Temperature stress/Antioxidation/Digestive enzymes分类
农业科技引用本文复制引用
刘玲,陈超,李炎璐,刘莉,陈建国,李文升,马文辉..短期温度胁迫对驼背鲈(♀)×鞍带石斑鱼((♂))杂交子代幼鱼抗氧化及消化酶活性的影响[J].渔业科学进展,2018,39(2):59-66,8.基金项目
山东省自然科学基金项目(ZR2015PC015)和中国-东盟海上合作基金共同资助[This work was supported by Natural Science Foundation of Shandong Province (ZR2015PC015),and China ASEAN Maritime Cooperation Fund Project]. （ZR2015PC015）
