渔业科学进展2018，Vol.39Issue(2)：67-75,9.DOI:10.19663/j.issn2095-9869.20171205001
云纹石斑鱼(♀)×鞍带石斑鱼((♂))杂交后代染色体核型分析
Chromosome Karyotype Analysis of Hybrids of Epinephelus moara ♀ × Epinephelus lanceolatus(♂)
摘要
Abstract
In this research,the chromosomal preparation and karyotype of Epinephelus moara♀ ×Epinephelus lanceolatus(♂) hybrid progeny (commonly known as Yunlong grouper) were studied.The juvenile fish weighing 60～100 g were injected subcutaneously with 6 μg/g phytohemagglutinin (PHA) and 0.1‰ colchicine.After 21 h,the head kidney and gill were dissected for cell suspensions,slices of their chromosome split phase were made by cold and hot drop method combing with air-drying.After being stained with 10％ Giemsa,a clear and complete split phase was screened under a microscope.Chromosomes in 75 mitotic phases were selected and the karyotype analysis was performed.The results showed that the karyotype formula was determined to be 2n=48,4sm+6st+38t,NF=58 in Yunlong grouper.Hybrids Yunlong grouper had the same chromosome number with their parents (2n=48),but their karyotype was different,indicating the complex chromosomal recombination and mutation occurred in the genetic process.This study provided an important scientific basis for the germplasm identification genetic relationship analysis and crossbreeding of Yunlong grouper.关键词
云纹石斑鱼/鞍带石斑鱼/云龙斑/染色体PHA注射法/核型分析Key words
Epinephelus moara/Epinephelus lanceolatus/Yunlong grouper/Chromosome PHA injection method/Karyotype analysis分类
农业科技引用本文复制引用
成美玲,翟介明,李波,田永胜,吴玉萍,李振通,张晶晶,王林娜,李文升,马文辉,王晓梅..云纹石斑鱼(♀)×鞍带石斑鱼((♂))杂交后代染色体核型分析[J].渔业科学进展,2018,39(2):67-75,9.基金项目
山东省农业良种工程(2016LZGC009)、烟台市科技发展计划(2016JH021)、烟台市“双百计划”人才项目和黄海水产研究所基本科研业务费(20603022017013)共同资助[The work was supported by Shandong Province Agricultural Variety Engineering (2016LZGC009),Yantai Science and Technology Development Plan(2016JH021),the Double-Hundred Talent Project of Yantai City,Shandong Province,and Special Fund for Basic Scientific Research Business of Central Public Research Institutes (20603022017013)] （2016LZGC009）
