渔业科学进展2018，Vol.39Issue(2)：120-127,8.DOI:10.19663/j.issn2095-9869.20170216001
不同温度下凡纳滨对虾和中国明对虾对白斑综合征病毒(WSSV)耐受性比较
The Difference of Tolerance to White Spot Syndrome Virus Between Litopenaeus vannamei and Fenneropenaeus chinensis at Different Temperatures
摘要
Abstract
The present study evaluated the effects of white spot syndrome virus (WSSV) infection in Litopenaeus vannamei ‘Renhai No.1’ and Fenneropenaeus chinensis ‘Huanghai No.2’ at various temperatures (24℃,28℃,and 32℃) using quantitative feeding method (L represents L.vannamei,and F represents F.chinensis).The results showed that the average survival time of group L-24℃,F-24℃,L-28 ℃,and F-28℃ was (184.05±69.56) h,(101.68±38.45) h,(100.25±26.79) h,and (73.38±22.22) h,respectively.The survival time between two kinds of shrimps at same temperature was significantly different (P＜0.05).At the 15th d,the survival rate of group L-32℃ and F-32℃ was 45.74％ and 23.47％,respectively.The time of 50％ mortality rate of two shrimps at three temperatures was 178 h and 98 h,98 h and 74 h,292 h and 78 h,respectively.The time of death peak of two shrimps among three temperatures was 5 d and 4 d,5 d and 4 d,10 d and 4 d,respectively.The virus loads in muscle of L-24℃ and F-24℃ at 144 h was (2.97× 106±7.44× 106) and (8.08× 106±3.22× 106) copies/ng DNA with the highly significant difference (P＜0.01).The virus loads in muscle of L-28℃ and F-28℃ at 144h was (6.73×106±1.49×106) and (1.20×107±6.15×105) copies/ng DNA with highly significant difference (P＜0.01).The virus loads of muscle of L-32℃ and F-32℃ at the 15th d was (5.18×103±4.32×103) and (3.78× 104±8.97×(103) copies/ng DNA with a significant difference (P＜0.05).These results indicates that L.vannamei are more tolerant to WSSV infection than F..chinensis,and the ability of multiplication of WSSV in muscle at different temperatures was 28 ℃ ＞24℃ ＞32 ℃.关键词
凡纳滨对虾/中国明对虾/不同温度/白斑综合征病毒(WSSV)/耐受性Key words
Litopenaeus vannamei/Fenneropenaeus chinensis/Different temperatures/White spot syndrome virus (WSSV)/Tolerance分类
农业科技引用本文复制引用
冯亚萍,董丽君,孟宪红,孔杰,罗坤,栾生,李旭鹏,史晓丽,曹家旺,王明珠,王军..不同温度下凡纳滨对虾和中国明对虾对白斑综合征病毒(WSSV)耐受性比较[J].渔业科学进展,2018,39(2):120-127,8.基金项目
中国水产科学研究院黄海水产研究所基本科研业务费(20603022017001)、国家自然科学基金(41676148)、泰山学者良种工程项目和现代农业产业技术体系专项资金(CARS-48)共同资助[This work was supported by Central Public-Interest Scientific Institution Basal Research Fund,Yellow Sea Fisheries Research Institute,CAFS(20603022017001),National Natural Science Foundation of China (41676148),Taishan Scholar Program for Seed Industry,and China Agriculture Research System (CARS-48)]. （20603022017001）
