渔业科学进展2018，Vol.39Issue(2)：177-182,6.DOI:10.19663/j.issn2095-9869.20170111001
海参中单糖检测方法的建立及含量测定
Establishment and Determination of Monosaccharide in Sea Cucumber
摘要
Abstract
High-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD) was widely used to analyze monosaccharide in botanical and fungal polysaccharides,but lightly used in marine animal polysaccharides.A method has been developed for determining monosaccharide composition and content in sea cucumber polysaccharide by HPAEC-PAD.Purifying sea cucumber polysaccharide were optimized by comparing three deproteinization methods.Optimum chromatographic conditions for the separation of monosaccharide were optimized by comparing eluent concentration on the separation of monosaccharide.The results indicated that the reagent of Sevag removed micro-molecule polypeptide more effectively than others from sea cucumber polysaccharides.The concentration of eluent had significant effect on separating monosaccharide.Eight monosaccharide,such as Fuc,Gal,Ara,GalN,GlcN,Man,Glc,and Lac,were separated using 20 mmol/L NaOH as eluent.GlcUA and GalUA were separated by 160 mmol/L NaOH and 200 mmol/L NaAC as eluent.The linear coefficients of monosaccharide standard were above 0.998.The detection limit of each monosaccharide was between 2.5 and 50 μg/L,and the recovery rates ranged from 83.6％ to 113.1％.The method determining neutral sugar,amino sugar and uronic acid in sea cucumber polysaccharides could be used as analysis and quantitative method of monosaccharide in sea cucumber and its products.The composition and content of monosaccharide were significant indices for determining the biological activity of sea cucumber.The established method could provide basic monosaccharide information and promote the polysaccharides research progress in sea cucumber.It could provide references for the establishment of sea cucumber standards,and expand the application of active substances in medicine and other fields.关键词
高效阴离子交换-脉冲安培检测法/海参多糖/单糖测定Key words
HPAEC-PAD/Sea cucumber polysaccharide/Monosaccharide determination分类
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刘芬,孙晓杰,朱文嘉,郭莹莹,何柳,杨祯祯,王媛媛,文艺晓,王联珠..海参中单糖检测方法的建立及含量测定[J].渔业科学进展,2018,39(2):177-182,6.基金项目
农业行业标准制定与修订(农产品质量安全)项目(2130109201515026-15)资助[This work was supported by Agricultural Industry Standard Setting and Revision Program (Quality and Safety of Agricultural Products)(2130109201515026-15)]. （农产品质量安全）
