临床口腔医学杂志2018，Vol.34Issue(4)：195-199,5.DOI:10.3969/j.issn.1003-1634.2018.04.002
人脱落乳牙牙髓干细胞促进破骨细胞形成的体外实验研究
The study of stem cells from human exfoliated deciduous teeth promote osteoclastogenesis in vitro
摘要
Abstract
Objective:To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED)on dif-ferentiation of osteoclast precursors in vitro by using indirect co-culture approach.Methods:The indirect co-culture system of SHED and osteoclast precursor cell line peripheral blood mononuclear cells(PBMCs)was established.In order to evaluate the osteoclastic differentiation of PBMCs after co-culture,the morphology of the osteoclast-like cells was observed by TRAP stai-ning and the number of TRAP+cells was counted.The expression of TRAP,CTSK,c-Fos,TRAF6 in PBMCs was evaluated by qPCR and Western Blot.Results:SHED promoted the differentiation of PBMCs into TRAP+osteoclast-like cells and the expression of TRAP,CTSK,c-Fos,TRAF6 in PBMCs,compared with control group.Conclusion:SHED can promote the dif-ferentiation of osteoclast precursors into osteoclast-like cells by noncontact co-culture.关键词
人脱落乳牙牙髓干细胞/外周血单个核细胞/间接共培养/破骨细胞分化Key words
Stem cells from human exfoliated deciduous teeth(SHED)/Peripheral blood mononuclear cells/Indirect co-culture/Osteoclastic differentiation分类
生物科学引用本文复制引用
王宇琛,王辰,刘娜,李伟,徐璐璐..人脱落乳牙牙髓干细胞促进破骨细胞形成的体外实验研究[J].临床口腔医学杂志,2018,34(4):195-199,5.基金项目
科技部国际科技合作项目(2015-TSYS-2028) （2015-TSYS-2028）
国家自然科学基金(81701018) （81701018）
解放军总医院临床科研扶持基金(2017FC-TSYS-3013) （2017FC-TSYS-3013）
