临床口腔医学杂志2018，Vol.34Issue(4)：209-212,4.DOI:10.3969/j.issn.1003-1634.2018.04.006
LPS对人牙髓干细胞增殖及骨向分化能力的影响
The effect of LPS on the proliferation and osteogenic differentiation of human dental pulp stem cells
摘要
Abstract
Objective:To explore the effect of lipopolysaccharide(LPS)in the regulation of proliferation and osteo-genic differentiation of human dental pulp stem cells.Methods:The third generation of human dental pulp stem cells were cultured in vitro and treated with LPS for 72 hours at different concentrations of LPS(0,1,10,100 and 1000 ng/mL)respec-tively.MTT assay was employed to evaluate the optimum concentration of LPS for promotion cell proliferati on.The protein ex-pression level of ALP,OCN,BSP and DSPP in hDPSCs treated with low concentration of LPS were examined respectively by Western Blot.Results:The proliferation of hDPSCs were obviously induced by low concentration of LPS(1 ng/mL and 10 ng/mL),and 10 ng/mL LPS was better to promote cell proliferation.Western Blot results showed that 10 ng/mL LPS up-regula-ted expression of osteogenic differentiation related proteins.Conclusion:LPS can enhance proliferation and osteogenic differ-entiation of hDPSCs at low concentration.关键词
脂多糖(LPS)/人牙髓干细胞/增殖/分化Key words
Lipopolysaccharide(LPS)/Dental pulp stem cell/Proliferation/Differentiation分类
生物科学引用本文复制引用
施俊,宋雨红,贺维,冷卫东,彭兴春..LPS对人牙髓干细胞增殖及骨向分化能力的影响[J].临床口腔医学杂志,2018,34(4):209-212,4.基金项目
湖北医药学院研究生启动金计划项目(2011QDZR-21) （2011QDZR-21）
