摘要
Abstract
Objective To explore the GLS1 expression in gastric cancer tissues and its relationship with clinical prognosis.Methods A total of 88 samples of gastric mucosa cancer tissues and theiradjacent tumor mucosa epithelial tissueswere collectedfrom the patients with gastric carcinoma.The GLS1,Raptor,Rictor protein expressions were detected by immunohistochemistry,and the mRNA expressionsweretested by RT-PCR.The relationship betweenclinical pathological data and GLS1 expressionwere analysed.The patients were followed up for the survival rate.Results Frequencies of positiveexpressionsof GLS1(80.7%,71/88),Raptor(73.9%,65/88),and Rictor(88.6%,78/88) in gastric cancer tissuesweresignificantly higher than those in the adjacent tissues of 38.6%(34/88),23.9%(21/88),and 46.6% (41/88),respectively (P<0.05).Additionally,mRNA expressionsof GLS1,Raptor,and Rictor in the cancer tissueswere (1.04±0.21),(1.73±0.32),and (1.32±0.17),respectively,significantly higher (P<0.05)than those in adjacent tissues(0.65±0.09),(0.81±0.12),and(0.75±0.11).Patients with tumors of bigger than 5cm in diameter,low differentiation,III~IV stage,lymph node metastasis,and the depth of invasion between T3~T4 presented a significantly high GLS1 positivity (P<0.05).The three year cumulative survival rate of patients with negativeGLS1 was 78.1%,higher than 47.2%(P<0.05) in positiveGLS1 patients.Multivariate analysis showed that GLS1 expression,differentiation degree and TNM stage were independent risk factors for prognosis of patients with gastric cancer (P<0.05).Conclusion The expression level of GLS1 in gastric carcinoma are significantly elevated,and the high GLS1 expression is associated with the poor prognosis of the disease,suggesting that GLS1 may be used as an indicator for evaluating the prognosis of gastric cancer.关键词
谷氨酰胺酶1型/胃癌/癌旁组织/预后Key words
Glutaminase1/ Gastric cancer/ Adjacent tissue/ Prognosis分类
医药卫生
