临床输血与检验2018，Vol.20Issue(2)：206-208,3.DOI:10.3969/j.issn.1671-2587.2018.02.031
9例核酸反应性标本与对应ELISA、CLIA法检测HBV的关联分析
Correlation about the 9 Specimens of Nucleic Acid Reactive with the Corresponding ELISA Method,CLIA Method Detection of HBV
摘要
Abstract
Objective To ensure the safety of clinical blood transfusion,Comparison of known ELISA Detection,ALT negative combined with NAT detection of reactive specimens and the corresponding CLIA method to detect HBV were analyzed. The correlation of the three detection methods was analysed. Methods 17 508 cases of blood donors (excluding ones of the same positive in ELISA,ALT positive) were detected by NAT and CLIA. Results Nucleic acid was detected in 9 cases of DNA HBV reactive specimens,RNA HCV reactive specimens of 0 cases and 0 cases of RNA HIV reactive specimens. 1 case in ELISA method was "negative value" High specimens. In CLIA method HBV "two pairs of half"detection,7 cases of HBcAb reactivity,2 cases no response. Conclusion First,blood donors detect methods in NAT and ELISA method was complementary,NAT detection can make up the "window period" of ELISA method. Second,NAT and CLIA test results were consistent; Third,OBI may be the main types of HBV DNA NAT detected blood donors in Handan area. Fourth,to reduce blood transfusion infection risk,the "negative value" high specimen in ELISA,which affected the quality of blood,should not be used,关键词
核酸检测/ELISA法/CLIA法/HBVKey words
Nucleic acid detect/ELISA method/CLIA method/HBV分类
医药卫生引用本文复制引用
张毓,孙国栋,张丽,王学刚..9例核酸反应性标本与对应ELISA、CLIA法检测HBV的关联分析[J].临床输血与检验,2018,20(2):206-208,3.
