环境与职业医学2018，Vol.35Issue(3)：272-276,5.DOI:10.13213/j.cnki.jeom.2018.17553
骨化三醇对大鼠矽肺纤维化的干预作用及其机制
Intervention effect of calcitriol on silica-induced lung fibrosis in rats and relevant mechanism
徐忠波 1赵杰 2贾晓民 2杜永亮2
作者信息
- 1. 徐州医科大学研究生学院,江苏徐州221000
- 2. 徐州医科大学第二附属医院呼吸科,江苏徐州221000
- 折叠
摘要
Abstract
[Objective] To explore the intervention effect of calcitriol on silica-induced lung fibrosis in rats and its possible mechanism.[Methods] SD rats (n=48) were randomly divided into four groups:control group,model group,solvent group,and calcitriol group,with 12 rats in each group.Rats in the model group,solvent group,and calcitriol group were exposed to 1 mL silica particle suspension (50 mg/mL) intratracheally to establish silicosis rat model,while the control group was exposed to an equal volume of saline using the same protocol.Starting from the day of model establishment,the calcitriol group was given intragastric administration of castor oil-diluted calcitriol (0.5 μg/kg) daily,the solvent group was treated with an equal volume of castor oil,and the control group and the model group were given an equal volume of saline.Six rats of each group were sacrificed randomly at 14 and 28 days after modelling respectively.The pathological changes of lung tissues were observed by HE staining and Masson staining.The content of hydroxyproline was detected by alkaline hydrolysis.The expression of transforming growth factor-β1 (TGF-β1),SMA-and MAD-related protein (SMAD) 3,and SMAD7 in lung tissues were detected by immunohistochemistry.[Results] The calcitriol group showed alleviative lung inflammation and interstitial fibrosis than the model group.Compared with the model group,the pulmonary organ coefficients and hydroxyproline content were decreased in the calcitriol group (P＜0.05).The protein expressions of TGF-β 1 (0.0219 ± 0.003 1) and SMAD3 (0.036 9 ± 0.0016)in the calcitriol group were lower than those in the model group [(0.036 1 ± 0.002 2) and (0.0420 ± 0.0020)] (P ＜ 0.05),and the protein expression of SMAD7 (0.033 3 ± 0.002 1) in the calcitriol group was higher than that of the model group (0.014 4 ± 0.0024)(P ＜ 0.05).There were no significant differences in above indicators between the model group and the solvent group (P＞0.05).[Conclusion] Calcitriol could alleviate silicosis fibrosis in rats,which may be associated with its regulation of protein expression in TGF-β1/SMAD pathway.关键词
骨化三醇/矽肺纤维化/TGF-β1/SMAD3/SMAD7Key words
calcitriol/silicosis fibrosis/TGF-β1/SMAD3/SMAD7分类
医药卫生引用本文复制引用
徐忠波,赵杰,贾晓民,杜永亮..骨化三醇对大鼠矽肺纤维化的干预作用及其机制[J].环境与职业医学,2018,35(3):272-276,5.
