农业机械学报2018，Vol.49Issue(3)：235-242,8.DOI:10.6041/j.issn.1000-1298.2018.03.028
生物炭对华北冬小麦根系形态和内生真菌多样性的影响
Effect of Biochar on Root Morphology and Endophytic Fungal Diversity of Winter Wheat in North China
摘要
Abstract
The winter wheat was selected to study the effect of biochar of cotton straw on root morphology and endophytic fungal communities by using the method of root scanning and Illumina Miseq highthroughput sequencing.The symbiosis mechanism was found by the test.The treatments were amount of 4.5 t/hm2,9.0 t/hm2 and 13.5 t/hm2 of straw biochar,respectively applied to the soil,and the straw returning was taken as test control in the field experiment.The results showed that root diameter and specific root length were increased significantly,and branching density and biomass were decreased significantly in the primary root,and only the root branching density was decreased in the secondary root,when the amount of biochar was 4.5 ～ 13.5 t/hm2 in the mature period of winter wheat.At the fungal phyla level,the abundance of Ascomycota was increased significantly,and the abundances of Zygomycota,Chytridiomycota and unidentified fungus was reduced greatly,and the abundance of Glomeromycota was not significant.At the common fungal order level,the abundance of Pleosporales was increased significantly,and the abundance of Eurotiales and Mortierellales was reduced greatly.With the amount of 4.5 ～ 9.0 t/hm2 biochar promoted the primary root growth of root branch density and biomass by decreasing the abundance of Eurotiales,and inhibited the secondary root growth of diameter and branch density by increasing the abundance of Pleosporales.By comprehensive comparison,the effect of amount of 9.0 t/hm2 biochar on root morphology was better than that of other treatments.The treatment with amount of 9.0 t/hm2 significantly increased the primary root diameter by 5.00％ and specific root length by 33.57％,and decreased root branch density by 67.26％ and biomass by 27.27％,while also reduced the secondary root diameter by 13.16％ and branch density by 34.38％,compared with the test control at maturity stage of winter wheat.关键词
冬小麦/生物炭/初生根/次生根/内生真菌/多样性Key words
winter wheat/biochar/primary root/secondary root/endophytic fungus/diversity分类
农业科技引用本文复制引用
李瑞霞,李洪杰,霍艳丽,高悦,杨正礼,张爱平..生物炭对华北冬小麦根系形态和内生真菌多样性的影响[J].农业机械学报,2018,49(3):235-242,8.基金项目
公益性行业(农业)科研专项(201503106)和张家口市科学技术研究与发展计划项目(1611051C) （农业）
