农业机械学报2018，Vol.49Issue(3)：322-327,6.DOI:10.6041/j.issn.1000-1298.2018.03.039
两步预处理对厌氧消化产酸纤维酶解和沼气发酵的影响
Effect of Two-step Pretreatment on Enzymatic Hydrolysis and Biogas Fermentation of Anaerobically Digested Fiber from VFAs Production
摘要
Abstract
The enzymatic hydrolysis and biogas fermentation of anaerobically digested fiber from volatile fatty acids (VFAs) production with rice straw were studied.The effects of two-step pretreatment of acetic acid and aqueous ammonia on the degradation of lignocellulose,glucose concentration,xylose concentration and biogas production were explored.The results showed that two-step pretreatment of acetic acid and aqueous ammonia can further degrade the hemicellulose and lignin in anaerobically digested fiber from VFAs production,and the recovery rate of cellulose was higher,which was 77.47％ ～81.44％.Among them,the mass fraction of cellulose was 56.82％ and the mass fraction of hemicellulose was only 7.94％ for pretreatment of 4％ acetic acid and 10％ aqueous ammonia at 121℃.Under the above pretreatment conditions,the enzymatic saccharification efficiency was the highest,the mass concentration of glucose was 47.46 g/L,and the glucose yield was 67.73％.Furthermore,the biogas production rate of anaerobically digested fiber from volatile fatty acids production pretreated by twostep pretreatment was better than those of acetic acid pretreatment alone and aqueous ammonia pretreatment alone.The highest biogas yield (414 mL/g) was obtained.Two-step pretreatment effectively improved the biogas yield of anaerobically digested fiber from VFAs production.Therefore,the research proved a new biorefinery way of anaerobically digested fiber.关键词
厌氧消化产酸纤维/酶解/沼气发酵/两步预处理Key words
anaerobically digested fiber from VFAs production/enzymatic hydrolysis/biogas fermentation/two-step pretreatment分类
资源环境引用本文复制引用
王殿龙,吴波,艾平,辛娅,何明雄,张衍林..两步预处理对厌氧消化产酸纤维酶解和沼气发酵的影响[J].农业机械学报,2018,49(3):322-327,6.基金项目
国家自然科学基金项目(51406064)、中国农业科学院基本科研业务费前沿探索项目(Y2017JC39)、四川省科技支撑计划项目(17ZDYF1305)和农业部沼气科学研究所基本科研业务费项目(1610012016020) （51406064）
