农业机械学报2018，Vol.49Issue(3)：354-360,7.DOI:10.6041/j.issn.1000-1298.2018.03.044
溶酶体组织蛋白酶参与宰后牛肉AIF介导细胞凋亡研究
Lysosomal Cathepsins in Apoptosis-inducing-factor-mediate Apoptosis of Bovine Muscle during Postmortem Aging
摘要
Abstract
With the aim to investigate apoptosis-inducing-factor (AIF)-mediate apoptosis and mechanism of lysosomal cathepsins involved in AIF activation of bovine muscle during postmortem aging,six healthy Simmental crossbreeding local yellow cattle were slaughtered and longissimus thoracic muscles were removed from the carcasses then aged at 4℃ for 6 h,12 h,24 h,72 h,120 h and 168 h.At the end of each storage period,ROS content,lysosomal membrane stability,cathepsins activities,mitochondrial membrane permeability,the expression of AIF and nuclear morphology were measured.The results suggested that ROS content was decreased from 0 h to 6 h,but it was significantly increased from 6 h to 168 h during postmortem aging (P ＜ 0.05).Lysosomal membrane stability was significantly decreased from 0 h to 168 h (P ＜0.05).Cathepsin B,D and L activities were increased as the postmortem aging was increased (P ＜0.05).Mitochondrial membrane permeability was significantly increased during postmortem aging (P ＜ 0.05).The AIF level in mitochondria was significantly increased from 0 h to 12 h and decreased as the postmortem aging was increased,the amount of AIF in the nucleus was increased during postmortem aging (P ＜0.05).The volume of cell nucleus was gradually increased and some nucleus were dissolved and broken during bovine postmortem aging.It was concluded that the generation of ROS may lead to imbalance of lysosomal membrane stability,and then cathepsins were released to cytoplasm.Subsequently,the mitochondrial outer membrane was permeabilized,and AIF was translocated to the cytosol and nucleus,where it induced apoptosis of nucleus.关键词
牛肉/宰后成熟/溶酶体组织蛋白酶/凋亡诱导因子/细胞凋亡Key words
bovine/postmortem aging/lysosomal cathepsins/apoptosis-inducing-factor/apoptosis分类
轻工纺织引用本文复制引用
张佳莹,余群力,韩玲,李航,殷元虎,韩明山..溶酶体组织蛋白酶参与宰后牛肉AIF介导细胞凋亡研究[J].农业机械学报,2018,49(3):354-360,7.基金项目
国家自然科学基金项目(31560463、31460402)和国家肉牛牦牛产业技术体系项目(CARS-38) （31560463、31460402）
